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Toxins 2018, 10(7), 263; https://doi.org/10.3390/toxins10070263

Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method

1
Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan
2
Department of Environmental and Food Sciences, Faculty of Environmental and Information Sciences, Fukui University of Technology, 3-6-1 Gakuen, Fukui, Fukui 910-8505, Japan
3
International Maize and Wheat Improvement Center (CIMMYT), Apdo, Postal 6-641, Mexico City 06600, Mexico
*
Author to whom correspondence should be addressed.
Received: 2 June 2018 / Revised: 24 June 2018 / Accepted: 25 June 2018 / Published: 27 June 2018
(This article belongs to the Collection Aflatoxins)
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Abstract

The dichlorvos–ammonia (DV–AM) method is a sensitive method for distinguishing aflatoxigenic fungi by detecting red (positive) colonies. In this study, the DV–AM method was applied for the isolation of aflatoxigenic and atoxigenic fungi from soil samples from a maize field in Mexico. In the first screening, we obtained two isolates from two soil subsamples of 20 independent samples and, in the second screening, we obtained two isolates from one subsample of these. Morphological and phylogenic analyses of the two isolates (MEX-A19-13, MEX-A19-2nd-5) indicated that they were Aspergillus flavus located in the A. flavus clade. Chemical analyses demonstrated that one isolate could produce B-type aflatoxins, while the other produced no aflatoxins. These results demonstrate that the DV–AM method is useful for the isolation of both aflatoxigenic and atoxigenic Aspergilli. View Full-Text
Keywords: aflatoxin B1; aflatoxin B2; fungal strain aflatoxin B1; aflatoxin B2; fungal strain
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Kushiro, M.; Hatabayashi, H.; Yabe, K.; Loladze, A. Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method. Toxins 2018, 10, 263.

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