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A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs

SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, Key Laboratory of Food Safety Research, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200000, China
Academy of State Administration of Grain P.R.C, No. 11 Baiwanzhuang Avenue, Xicheng District, Beijing 100037, China
Key Laboratory of Agro-Products Quality and Safety of Xinjiang/Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture/Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Laboratory of Food Analysis, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium
Authors to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
Toxins 2018, 10(3), 129;
Received: 6 February 2018 / Revised: 14 March 2018 / Accepted: 15 March 2018 / Published: 20 March 2018
(This article belongs to the Section Mycotoxins)
PDF [1238 KB, uploaded 3 May 2018]


The determination of zearalenone (ZEN) and its derivatives as biomarkers in animal tissues or organs plays an important role in mycotoxin monitoring and can promote effective exposure assessment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of nine ZEN-like mycotoxins, including three glucuronides in different pig tissues (heart, liver, spleen and muscle) was developed and validated in this study. Tissue samples were extracted using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and clean-up procedure, and analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode. Dynamic linear ranges for each target analyte were determined with R2 between 0.916 and 0.999. The LODs of the six ZENs were achieved in the range of 0.5–1 ng/g and the LOQs varied from 1 ng/g to 2 ng/g. The satisfying intra-day and inter-day reproducibility (both RSDr and RSDR < 20%) indicated a good stability of this method. The recoveries of the nine target analytes were in the range of 70–110%. The validation results showed that this LC-MS/MS method coupled with QuEChERS sample pretreatment is effective and suitable for the simultaneous quantitation of ZEN metabolites in pigs. It has been applied to analysis of the pig tissues in this research and can be also adapted for samples in the mycotoxin research field. View Full-Text
Keywords: zearalenone; LC-MS/MS; QuEChERS; pigs zearalenone; LC-MS/MS; QuEChERS; pigs

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Yan, Z.; Wang, L.; Wang, J.; Tan, Y.; Yu, D.; Chang, X.; Fan, Y.; Zhao, D.; Wang, C.; De Boevre, M.; De Saeger, S.; Sun, C.; Wu, A. A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs. Toxins 2018, 10, 129.

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