Zeolite-Containing Mixture Supplementation Ameliorated Dextran Sodium Sulfate-Induced Colitis in Mice by Suppressing the Inflammatory Bowel Disease Pathway and Improving Apoptosis in Colon Mucosa

Corporate Sponsored Research Program “Food for Life”, Organization for Interdisciplinary Research Projects, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

Azuma Chemical Co. Ltd., 3-4-4 Iidabashi, Chiyoda-ku, Tokyo 102-0072, Japan

Authors to whom correspondence should be addressed.

Nutrients 2017, 9(5), 467; https://doi.org/10.3390/nu9050467

Received: 17 February 2017 / Revised: 28 April 2017 / Accepted: 2 May 2017 / Published: 6 May 2017

(This article belongs to the Special Issue Selected Papers from APNNO Biennial Conference 2016)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Inflammatory bowel disease (IBD) is induced by multiple environmental factors, and there is still no known treatment capable of curing the disease completely. We propose a zeolite-containing mixture (Hydryeast^®, HY)—a multi-component nutraceutical of which the main ingredients are Azumaceramics (mixture of zeolite and oyster shell burned under high temperature), citric acid, red rice yeast (monascus) and calcium stearate—as a nutraceutical intervention in IBD to ameliorate dextran sodium sulfate (DSS)-induced colitis. We show the mechanism through integrated omics using transcriptomics and proteomics. C57BL6 mice were given an AIN-93G basal diet or a 0.8% HY containing diet and sterilized tap water for 11 days. Colitis was then induced by 1.5% (w/v) DSS-containing water for 9 days. HY fed mice showed significantly improved disease activity index and colon length compared to DSS mice. Colonic mucosa microarray analysis plus RT-PCR results indicate HY supplementation may ameliorate inflammation by inhibiting the intestinal inflammatory pathway and suppress apoptosis by curbing the expression of genes like tumor protein 53 and epidermal growth factor receptor and by upregulating epithelial protection-related proteins such as epithelial cell adhesion molecule and tenascin C, thus maintaining mucosal immune homeostasis and epithelial integrity, mirroring the proteome analysis results. HY appears to have a suppressive effect on colitis. View Full-Text

Keywords: zeolite-containing mixture (Hydryeast^®); Azumaceramics; DSS; inflammatory bowel disease; apoptosis; colon mucosa

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

Supplementary File 1:
XLSX-Document (XLSX, 544 KiB)

Share and Cite

MDPI and ACS Style

Lyu, W.; Jia, H.; Deng, C.; Saito, K.; Yamada, S.; Kato, H. Zeolite-Containing Mixture Supplementation Ameliorated Dextran Sodium Sulfate-Induced Colitis in Mice by Suppressing the Inflammatory Bowel Disease Pathway and Improving Apoptosis in Colon Mucosa. Nutrients 2017, 9, 467. https://doi.org/10.3390/nu9050467

AMA Style

Lyu W, Jia H, Deng C, Saito K, Yamada S, Kato H. Zeolite-Containing Mixture Supplementation Ameliorated Dextran Sodium Sulfate-Induced Colitis in Mice by Suppressing the Inflammatory Bowel Disease Pathway and Improving Apoptosis in Colon Mucosa. Nutrients. 2017; 9(5):467. https://doi.org/10.3390/nu9050467

Chicago/Turabian Style

Lyu, Weida, Huijuan Jia, Chuanzong Deng, Kenji Saito, Seigo Yamada, and Hisanori Kato. 2017. "Zeolite-Containing Mixture Supplementation Ameliorated Dextran Sodium Sulfate-Induced Colitis in Mice by Suppressing the Inflammatory Bowel Disease Pathway and Improving Apoptosis in Colon Mucosa" Nutrients 9, no. 5: 467. https://doi.org/10.3390/nu9050467

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Zeolite-Containing Mixture Supplementation Ameliorated Dextran Sodium Sulfate-Induced Colitis in Mice by Suppressing the Inflammatory Bowel Disease Pathway and Improving Apoptosis in Colon Mucosa

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI