Bioavailability of Zinc in Wistar Rats Fed with Rice Fortified with Zinc Oxide
Abstract
:1. Introduction
2. Experimental Section
2.1. Raw Material
2.2. Experimental Diets
Components * | Test Diets | Control Diet | ||
---|---|---|---|---|
R15 (g/100 g) | R30 (g/100 g) | C15 (g/100 g) | C30 (g/100 g) | |
ZnCO3 (mg) | - | - | 0.002876 | 0.005752 |
Dietary iron (ferric pyrophosphate) | 0.0059 | 0.0059 | 0.0059 | 0.0059 |
Ultra Rice® a | 1.14 | 2.28 | - | - |
Albumin ** | 19.91 | 19.83 | 20.00 | 20.00 |
Maltodextrin ** | 13.20 | 13.20 | 13.20 | 13.20 |
Sucrose ** | 10.00 | 10.00 | 10.00 | 10.00 |
Soybean oil ** | 6.94 | 6.87 | 7.00 | 7.00 |
Fiber (microfine cellulose) ** | 4.98 | 4.98 | 5.00 | 5.00 |
Mineral mix without zinc ** | 3.50 | 3.50 | 3.50 | 3.50 |
Vitamin mix ** | 1.00 | 1.00 | 1.00 | 1.00 |
l-cystine ** | 0.30 | 0.30 | 0.30 | 0.30 |
Choline bitartrate ** | 0.25 | 0.25 | 0.25 | 0.25 |
Corn starch ** | 38.77 | 37.79 | 39.75 | 39.75 |
Caloric density (kcal/g) | 3.69 | 3.69 | 3.78 | 3.78 |
2.3. Determination of Zn in UR® and in the Experimental Diets
2.4. Determination of the Bioavailability of Zn in Vivo
2.5. Chemical and Biochemical Analyses
2.6. Mineral Retention
2.7. Experimental Design and Analysis of the Data
3. Results and Discussion
3.1. Concentration of Zinc in the Experimental Diets
3.2. Biological Assay
Source | WG (g) | FI (g) | ZnI (mg) | FER (%) |
---|---|---|---|---|
ZnCO3 | 147.83 ± 22.31 a | 819.83 ± 60.34 a | 17.61 ± 8.18 a | 18.71 ± 3.69 a |
UR® | 124.12 ± 23.53 b | 798.80 ± 60.68 a | 18.49 ± 5.11 a | 15.39 ± 3.87 b |
Source | Zn-Femur (mg/g) | FW (g) | FL (cm) | FW (cm) |
---|---|---|---|---|
ZnCO3 | 0.11 ± 0.02 a | 0.80 ± 0.13 b | 3.14 ± 0.07 a | 0.30 ± 0.01 a |
UR® | 0.09 ± 0.01 b | 0.89 ± 0.11 a | 3.11 ± 0.13 a | 0.30 ± 0.01 a |
Source | ZnPlas (μg/mL) | ZnEryt (μg/gHb) |
---|---|---|
UR® | 77.71 ± 25.17 a | 32.09 ± 12.92 a |
ZnCO3 | 98.05 ± 37.66 a | 29.68 ± 17.79 a |
Source × Dose | RZn-Femur (mg/100 g) | RCa-Femur (mg/100 g) | RMg-Femur (mg/100 g) |
---|---|---|---|
C30 | 1.06 ± 0.25 a | 4.35 ± 1.56 a | 0.35 ± 0.08 a |
R30 | 0.63 ± 0.12 b | 3.40 ± 0.93 a | 0.33 ± 0.10 a |
C15 | 0.54 ± 0.23 c | 4.12 ± 1.12 a | 0.34 ± 0.10 a |
R15 | 0.46 ± 0.15 c | 3.52 ± 1.25 a | 0.32 ± 0.07 a |
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Della Lucia, C.M.; Santos, L.L.M.; Da Cruz Rodrigues, K.C.; Da Cruz Rodrigues, V.C.; Martino, H.S.D.; Sant'Ana, H.M.P. Bioavailability of Zinc in Wistar Rats Fed with Rice Fortified with Zinc Oxide. Nutrients 2014, 6, 2279-2289. https://doi.org/10.3390/nu6062279
Della Lucia CM, Santos LLM, Da Cruz Rodrigues KC, Da Cruz Rodrigues VC, Martino HSD, Sant'Ana HMP. Bioavailability of Zinc in Wistar Rats Fed with Rice Fortified with Zinc Oxide. Nutrients. 2014; 6(6):2279-2289. https://doi.org/10.3390/nu6062279
Chicago/Turabian StyleDella Lucia, Ceres Mattos, Laura Luiza Menezes Santos, Kellen Cristina Da Cruz Rodrigues, Vivian Cristina Da Cruz Rodrigues, Hércia Stampini Duarte Martino, and Helena Maria Pinheiro Sant'Ana. 2014. "Bioavailability of Zinc in Wistar Rats Fed with Rice Fortified with Zinc Oxide" Nutrients 6, no. 6: 2279-2289. https://doi.org/10.3390/nu6062279