Beneficial Effects of Milk-Derived Extracellular Vesicles on Liver Fibrosis Progression by Inhibiting Hepatic Stellate Cell Activation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Milk Sample Collection
2.2. Extracellular Vesicles’ Isolation from Milk
2.3. Nanoparticle Analysis
2.4. Dynamic Light Scattering
2.5. HSCs’ Isolation and Cell Culture
2.6. MDEs’ Feeding by Gavage
2.7. Exosomes Labeling
2.8. Cell Proliferation Assessment
2.9. Immunoblotting
2.10. RNA Extraction
2.11. mRNA Detection by qRT-PCR
2.12. MicroRNA Detection by qRT-PCR
2.13. Ethical Approval Information
3. Results
3.1. Isolation and Characterization of Milk-Derived Extracellular Vesicles
3.2. Uptake of MDEs In Vivo and In Vitro
3.3. MDEs Inhibit HSCs’ Proliferation
3.4. Downregulation of Collagen Expression and Upregulation of Peroxisome Proliferator-Activated Receptor—γ (PPAR-γ) by MDEs
3.5. Regulation of miRNA Expression by MDEs
3.6. The Effect of MDEs on HSCs In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Reif, S.; Atias, A.; Musseri, M.; Koroukhov, N.; Gerstl, R.G. Beneficial Effects of Milk-Derived Extracellular Vesicles on Liver Fibrosis Progression by Inhibiting Hepatic Stellate Cell Activation. Nutrients 2022, 14, 4049. https://doi.org/10.3390/nu14194049
Reif S, Atias A, Musseri M, Koroukhov N, Gerstl RG. Beneficial Effects of Milk-Derived Extracellular Vesicles on Liver Fibrosis Progression by Inhibiting Hepatic Stellate Cell Activation. Nutrients. 2022; 14(19):4049. https://doi.org/10.3390/nu14194049
Chicago/Turabian StyleReif, Shimon, Ariel Atias, Mirit Musseri, Nickolay Koroukhov, and Regina Golan Gerstl. 2022. "Beneficial Effects of Milk-Derived Extracellular Vesicles on Liver Fibrosis Progression by Inhibiting Hepatic Stellate Cell Activation" Nutrients 14, no. 19: 4049. https://doi.org/10.3390/nu14194049