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Open AccessArticle

Extensive Degradation and Low Bioavailability of Orally Consumed Corn miRNAs in Mice

1
Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, MD 20705, USA
2
Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(2), 215; https://doi.org/10.3390/nu10020215
Received: 23 January 2018 / Revised: 8 February 2018 / Accepted: 12 February 2018 / Published: 15 February 2018
The current study seeks to resolve the discrepancy in the literature regarding the cross-kingdom transfer of plant microRNAs (miRNAs) into mammals using an improved miRNA processing and detection method. Two studies utilizing C57BL/6 mice were performed. In the first study, mice were fed an AIN-93M diet and gavaged with water, random deoxynucleotide triphosphates (dNTP) or isolated corn miRNAs for two weeks (n = 10 per group). In the second study, mice were fed an AIN-93M diet, or the diet supplemented with 3% fresh or autoclaved corn powder for two weeks (n = 10 per group). Corn miRNA levels were analyzed in blood and tissue samples by real-time PCR (RT-PCR) following periodate oxidation and β elimination treatments to eliminate artifacts. After removing false positive detections, there were no differences in corn miRNA levels between control and treated groups in cecal, fecal, liver and blood samples. Using an in vitro digestion system, corn miRNAs in AIN-93M diet or in the extracts were found to be extensively degraded. Less than 1% was recovered in the gastrointestinal tract after oral and gastric phases. In conclusion, no evidence of increased levels of corn miRNAs in whole blood or tissues after supplementation of corn miRNAs in the diet was observed in a mouse model. View Full-Text
Keywords: corn; cross-kingdom transfer; C57BL/6 mouse; miRNA; periodate oxidation corn; cross-kingdom transfer; C57BL/6 mouse; miRNA; periodate oxidation
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Huang, H.; Davis, C.D.; Wang, T.T.Y. Extensive Degradation and Low Bioavailability of Orally Consumed Corn miRNAs in Mice. Nutrients 2018, 10, 215.

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