Next Article in Journal
Recent Perspectives Regarding the Role of Dietary Protein for the Promotion of Muscle Hypertrophy with Resistance Exercise Training
Previous Article in Journal
Predictors of Dietary Energy Density among Preschool Aged Children
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Nutrients 2018, 10(2), 179;

In Vivo Assessment of Resistant Starch Degradation by the Caecal Microbiota of Mice Using RNA-Based Stable Isotope Probing—A Proof-of-Principle Study

Institute of Precision Medicine, Faculty of Medical & Life Sciences, Furtwangen University, 78054 Villingen-Schwenningen, Germany
Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany
AgResearch Limited, Food Nutrition and Health Team, Grasslands Research Centre, Palmerston North 4474, New Zealand
Riddet Institute, Massey University, Palmerston North 4474, New Zealand
High-Value Nutrition, National Science Challenge, University of Auckland, Auckland 1142, New Zealand
The New Zealand Institute for Plant & Food Research Limited, Palmerston North 4474, New Zealand
Author to whom correspondence should be addressed.
Received: 22 November 2017 / Revised: 19 January 2018 / Accepted: 30 January 2018 / Published: 6 February 2018
Full-Text   |   PDF [2192 KB, uploaded 6 February 2018]   |  


Resistant starch (RS) is the digestion resistant fraction of complex polysaccharide starch. By reaching the large bowel, RS can function as a prebiotic carbohydrate, i.e., it can shape the structure and activity of bowel bacterial communities towards a profile that confers health benefits. However, knowledge about the fate of RS in complex intestinal communities and the microbial members involved in its degradation is limited. In this study, 16S ribosomal RNA (rRNA)-based stable isotope probing (RNA-SIP) was used to identify mouse bowel bacteria involved in the assimilation of RS or its derivatives directly in their natural gut habitat. Stable-isotope [U13C]-labeled native potato starch was administrated to mice, and caecal contents were collected before 0 h and 2 h and 4 h after administration. ‘Heavy’, isotope-labeled [13C]RNA species, presumably derived from bacteria that have metabolized the labeled starch, were separated from ‘light’, unlabeled [12C]RNA species by fractionation of isolated total RNA in isopycnic-density gradients. Inspection of different density gradients showed a continuous increase in ‘heavy’ 16S rRNA in caecal samples over the course of the experiment. Sequencing analyses of unlabeled and labeled 16S amplicons particularly suggested a group of unclassified Clostridiales, Dorea, and a few other taxa (Bacteroides, Turicibacter) to be most actively involved in starch assimilation in vivo. In addition, metabolic product analyses revealed that the predominant 13C-labeled short chain fatty acid (SCFA) in caecal contents produced from the [U13C] starch was butyrate. For the first time, this study provides insights into the metabolic transformation of RS by intestinal bacterial communities directly within a gut ecosystem, which will finally help to better understand its prebiotic potential and possible applications in human health. View Full-Text
Keywords: resistant starch; gut microbiota; RNA-SIP; Clostridiales; Dorea resistant starch; gut microbiota; RNA-SIP; Clostridiales; Dorea

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Herrmann, E.; Young, W.; Reichert-Grimm, V.; Weis, S.; Riedel, C.U.; Rosendale, D.; Stoklosinski, H.; Hunt, M.; Egert, M. In Vivo Assessment of Resistant Starch Degradation by the Caecal Microbiota of Mice Using RNA-Based Stable Isotope Probing—A Proof-of-Principle Study. Nutrients 2018, 10, 179.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top