Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains
Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano, 10, 85100 Potenza, Italy
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Nutrients 2009, 1(2), 210-223; https://doi.org/10.3390/nu1020210
Received: 17 September 2009 / Accepted: 25 November 2009 / Published: 30 November 2009
(This article belongs to the Special Issue Foodomics 2009)
Abstract
Lactobacillus sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors. We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, which allows the identification of gene expression changes associated with different microbial behaviours under different growth conditions. View Full-Text
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Bonomo, M.G.; Sico, M.A.; Grieco, S.; Salzano, G. Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains. Nutrients 2009, 1, 210-223.
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