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Article

Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid

1
Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China
2
National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, Huazhong Agricultural University, Wuhan 430070, China
3
College of Tropical Crops, Hainan University, Haikou 570228, China
4
Department of Agronomy, University of Haripur, Haripur 22620, Pakistan
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Academic Editors: Chunjiang An and Imre J. Holb
Sustainability 2021, 13(13), 6998; https://doi.org/10.3390/su13136998
Received: 6 May 2021 / Revised: 14 June 2021 / Accepted: 19 June 2021 / Published: 22 June 2021
Liquid-liquid extraction and solid phase extraction followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing the performance of the HPLC-UV method, including composition of the mobile phase for gradient elution, column temperature, flow rate, and detection wavelength, were optimized. This method can simultaneously determine 11 phytohormones, including nine cytokinins, indole-3-acetic acid, and abscisic acid. The limit of detection of this method is 0.22 to 1.1 µg L−1, and the coefficient factors of linear regression are >0.998. The recoveries of the target phytohormones ranged between 62.1~109.4%, and the relative standard deviations were <10%. This method is suitable for determining phytohormones, especially cytokinins, in young panicles, roots, and xylem sap of rice plants. View Full-Text
Keywords: HPLC-UV; cytokinin; indole-3-acetic acid; abscisic acid HPLC-UV; cytokinin; indole-3-acetic acid; abscisic acid
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MDPI and ACS Style

Qi, B.; Wu, C.; Liang, H.; Cui, K.; Fahad, S.; Wang, M.; Liu, B.; Nie, L.; Huang, J.; Tang, H. Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid. Sustainability 2021, 13, 6998. https://doi.org/10.3390/su13136998

AMA Style

Qi B, Wu C, Liang H, Cui K, Fahad S, Wang M, Liu B, Nie L, Huang J, Tang H. Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid. Sustainability. 2021; 13(13):6998. https://doi.org/10.3390/su13136998

Chicago/Turabian Style

Qi, Beibei, Chao Wu, Huiling Liang, Kehui Cui, Shah Fahad, Manlian Wang, Baoyu Liu, Lixiao Nie, Jianliang Huang, and Hui Tang. 2021. "Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid" Sustainability 13, no. 13: 6998. https://doi.org/10.3390/su13136998

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