Soil Physicochemical Properties, Metal Deposition, and Ultrastructural Midgut Changes in Ground Beetles, Calosoma chlorostictum, under Agricultural Pollution
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Sites and Sampling Procedure
2.2. The Insect Dissection
2.3. Physicochemical Parameters of Soil Samples
2.4. Analysis of Heavy Metal Concentrations in Soil Samples
2.5. Analysis of Heavy Metal Concentrations in the Midgut of C. chlorostictum Samples
2.6. Transmission Electron Microscopy (TEM) Examinations of the Midgut of C. chlorostictum Samples
2.7. Statistical Analysis
3. Results and Discussion
3.1. Physicochemical Parameters of the Soil Samples
3.2. Heavy Metal Concentrations in the Soil Samples
3.3. Heavy Metal Concentrations in the Midgut of C. chlorostictum
3.4. Ultrastructural Changes in the Midgut of C. chlorostictum Samples
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameters | Soil Samples | Independent T-test | ||
---|---|---|---|---|
Reference Site | Polluted Agri. Site | T-Statistic | p-Value | |
Conductivity (ds/m) | 6.271 ± 0.832 | 9.019 ± 1.007 | 7.288 * | ≤0.05 |
pH value | 8.355 ± 0.770 | 7.515 ± 0.683 | 2.827 * | ≤0.05 |
Moisture (%) | 1.956 ± 0.078 | 2.512 ± 0.096 | 15.571 * | ≤0.01 |
Chloride content (%) | 0.015 ± 0.081 | 0.018 ± 0.057 | 0.105 | >0.05 |
Total CaCO3 (%) | 2.891 ± 0.379 | 3.254 ± 0.402 | 2.276 * | ≤0.05 |
Organic matter (%) | 1.951 ± 0.208 | 1.034 ± 0.208 | 10.799 * | ≤0.01 |
Alkalinity (%) | 6.024 ± 0.735 | 4.825 ± 0.358 | 5.080 * | ≤0.05 |
Heavy Metals | Soil Samples (µg/g−1) | Independent T-Test | ||
---|---|---|---|---|
Reference Site | Polluted Agri. Site | T-Statistic | p-Value | |
Arsenic (As) | 9.075 ± 0.808 | 13.235 ± 1.014 | 11.115 * | ≤0.01 |
Mercury (Hg) | 0.808 ± 0.044 | 1.178 ± 0.113 | 10.570 * | ≤0.05 |
Aluminium (Al) | 2.780 ± 0.097 | 6.918 ± 0.475 | 29.568 * | ≤0.001 |
Cadmium (Cd) | 0.322 ± 0.046 | 0.905 ± 0.089 | 20.158 * | ≤0.01 |
Lead (Pb) | 0.918 ± 0.071 | 1.218 ± 0.095 | 8.762 * | ≤0.05 |
Selenium (Se) | 0.386 ± 0.043 | 0.512 ± 0.067 | 5.483 * | ≤0.05 |
Heavy Metals | Beetle Samples (µg/g−1) | Independent T-Test | ||
---|---|---|---|---|
Reference Site | Polluted Agri. Site | T-Statistic | p-Value | |
Arsenic (As) | 6.903 ± 0.628 | 11.858 ± 0.912 | 21.460 * | ≤0.01 |
Mercury (Hg) | 0.209 ± 0.036 | 0.630 ± 0.077 | 23.753 * | ≤0.01 |
Aluminium (Al) | 0.993 ± 0.077 | 2.768 ± 0.199 | 39.895 * | ≤0.001 |
Cadmium (Cd) | 0.278 ± 0.031 | 0.765 ± 0.069 | 30.876 * | ≤0.001 |
Lead (Pb) | 0.525 ± 0.045 | 0.849 ± 0.057 | 21.396 * | ≤0.01 |
Selenium (Se) | 0.201 ± 0.019 | 0.293 ± 0.036 | 10.839 * | ≤0.05 |
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Nasr, E.E.; Khater, Z.Z.; Zelenakova, M.; Vranayova, Z.; Abu-Hashim, M. Soil Physicochemical Properties, Metal Deposition, and Ultrastructural Midgut Changes in Ground Beetles, Calosoma chlorostictum, under Agricultural Pollution. Sustainability 2020, 12, 4805. https://doi.org/10.3390/su12124805
Nasr EE, Khater ZZ, Zelenakova M, Vranayova Z, Abu-Hashim M. Soil Physicochemical Properties, Metal Deposition, and Ultrastructural Midgut Changes in Ground Beetles, Calosoma chlorostictum, under Agricultural Pollution. Sustainability. 2020; 12(12):4805. https://doi.org/10.3390/su12124805
Chicago/Turabian StyleNasr, Enas E., Zeinab Z. Khater, Martina Zelenakova, Zuzana Vranayova, and Mohamed Abu-Hashim. 2020. "Soil Physicochemical Properties, Metal Deposition, and Ultrastructural Midgut Changes in Ground Beetles, Calosoma chlorostictum, under Agricultural Pollution" Sustainability 12, no. 12: 4805. https://doi.org/10.3390/su12124805