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Pediatric Reports
Volume 8
Issue 3
10.4081/pr.2016.6487
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Article

The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis

by
Francesca Tocchioni
1,
Chiara Tani
1,
Laura Bartolini
1,
Maria Moriondo
1,
Francesco Nieddu
3,
Patrizia Pecile
2,
Chiara Azzari
3,
Antonio Messineo
1 and
Marco Ghionzoli
1,*
1
Department of Pediatric Surgery, University of Florence and Children’s University Hospital A. Meyer, Italy
2
Department of Microbiology, Careggi Hospital, Florence, Italy
3
Department of Clinical Immunology, University of Florence and Children’s University Hospital A. Meyer, Italy
*
Author to whom correspondence should be addressed.
Pediatr. Rep. 2016, 8(3), 6487; https://doi.org/10.4081/pr.2016.6487
Submission received: 25 February 2016 / Revised: 4 July 2016 / Accepted: 29 July 2016 / Published: 19 September 2016

Abstract

Bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. DNA amplification represents a fast technique to detect microbial sequences. We aimed to compare the potential of DNA amplification versus traditional bacterial growth culture highlighting advantages and drawbacks in a surgical setting. Peritoneal fluid specimens were collected during surgery from 36 children who underwent appendectomy between May and December 2012. Real-time polymerase chain reaction (RT-PCR) and cultures were performed on each sample. RT-PCR showed an amplification of 16S in 18/36 samples, Escherichia coli (in 7 cases), Pseudomonas aeruginosa (3), Fusobacterium necrophorum (3), Adenovirus (2), E.coli (1), Klebsiella pneumoniae (1), Serratia marcescens/Enterobacter cloacae (1). Bacterial growth was instead observed only in four patients (3 E.coli and 1 P.aeruginosa and Bacteroides ovatus). Preoperative C-reactive protein and inflammation degree, the most reliable indicators of bacterial translocation, were elevated as expected. DNA amplification was a quick and useful method to detect pathogens and it was even more valuable in detecting aggressive pathogens such as anaerobes, difficult to preserve in biological cultures; its drawbacks were the lack of biological growths and of antibiograms. In our pilot study RT-PCR and cultures did not influence the way patients were treated.
Keywords: Antibiotics therapy; complicated appendicitis; microbiological culture; pathogens; Real-time-PCR. Antibiotics therapy; complicated appendicitis; microbiological culture; pathogens; Real-time-PCR.

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MDPI and ACS Style

Tocchioni, F.; Tani, C.; Bartolini, L.; Moriondo, M.; Nieddu, F.; Pecile, P.; Azzari, C.; Messineo, A.; Ghionzoli, M. The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis. Pediatr. Rep. 2016, 8, 6487. https://doi.org/10.4081/pr.2016.6487

AMA Style

Tocchioni F, Tani C, Bartolini L, Moriondo M, Nieddu F, Pecile P, Azzari C, Messineo A, Ghionzoli M. The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis. Pediatric Reports. 2016; 8(3):6487. https://doi.org/10.4081/pr.2016.6487

Chicago/Turabian Style

Tocchioni, Francesca, Chiara Tani, Laura Bartolini, Maria Moriondo, Francesco Nieddu, Patrizia Pecile, Chiara Azzari, Antonio Messineo, and Marco Ghionzoli. 2016. "The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis" Pediatric Reports 8, no. 3: 6487. https://doi.org/10.4081/pr.2016.6487

APA Style

Tocchioni, F., Tani, C., Bartolini, L., Moriondo, M., Nieddu, F., Pecile, P., Azzari, C., Messineo, A., & Ghionzoli, M. (2016). The Role of DNA Amplification and Cultural Growth in Complicated Acute Appendicitis. Pediatric Reports, 8(3), 6487. https://doi.org/10.4081/pr.2016.6487

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