Next Article in Journal
Molecular Investigation of Zoonotic Genotypes of Giardia intestinalis Isolates in Humans, Dogs and Cats, Sheep, Goats and Cattle in Araçatuba (São Paulo State, Brazil) by the Analysis of β-Giardin Gene Fragments
Previous Article in Journal
Antibacterial Activities of Serum from the Komodo Dragon (Varanus komodoensis)
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microbiology Research
Volume 4
Issue 1
10.4081/mr.2013.e5
microbiolres-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Microbiology Research is published by MDPI from Volume 11 Issue 2 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Cloning, Expression of the Major Capsid Protein Gene from Marine Algae Emiliania huxleyi Virus and the Possible Use in Detection of Virus Infection

by
Jingwen Liu
,
Zhilan Zhang
,
Xuhong Liu
,
Yiqin Cai
and
Huinong Cai
*
Bioengineering College of Jimei University, Xiame, China
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2013, 4(1), e5; https://doi.org/10.4081/mr.2013.e5
Submission received: 5 February 2013 / Revised: 23 April 2013 / Accepted: 15 May 2013 / Published: 9 September 2013

Abstract

Here we described the cloning, bioinformatic characterization and expression in Escherichia coli of the major capsid protein (MCP) from marine unicellular algae Emiliania huxleyi virus EhV-99B1 isolate. The purified recombinant MCP was used to develop a polyclonal antibody for testing viral infection. The full length open-reading frame (ORF) of MCP encodes a protein of 496 amino acids with a calculated molecular mass of 55 kDa and Ip 6.34. Hydropathy analysis of MCP showed that there were 6 largely hydrophobic domains, which may be important for the interaction with the envelope protein. The conserved region of EhV strains MCP had high similarity in amino acid sequence and secondary structure which allow us to develop a specific biomarker for EhVs infection detection. The full length ORF was subcloned into expression vector pGEX-4T-3 for overexpression in E. coli as glutathione-Stransferase-L1 (GST-L1) fusion protein and the soluble recombinant protein was used to generate polyclonal antibodies in mice. The obtained antisera reacted in Western immunoblots with the same protein both in purified EhV-99B1 virions and infected host cells sample. These shows that the antiserum against recombinant EhV-MCP offers the potential to develop immunofluorescence techniques for the detection of EhVs infected cells.
Keywords: Emiliania huxleyi virus; major capsid protein; bioinformatic characterization; anti-serum reaction Emiliania huxleyi virus; major capsid protein; bioinformatic characterization; anti-serum reaction

Share and Cite

MDPI and ACS Style

Liu, J.; Zhang, Z.; Liu, X.; Cai, Y.; Cai, H. Cloning, Expression of the Major Capsid Protein Gene from Marine Algae Emiliania huxleyi Virus and the Possible Use in Detection of Virus Infection. Microbiol. Res. 2013, 4, e5. https://doi.org/10.4081/mr.2013.e5

AMA Style

Liu J, Zhang Z, Liu X, Cai Y, Cai H. Cloning, Expression of the Major Capsid Protein Gene from Marine Algae Emiliania huxleyi Virus and the Possible Use in Detection of Virus Infection. Microbiology Research. 2013; 4(1):e5. https://doi.org/10.4081/mr.2013.e5

Chicago/Turabian Style

Liu, Jingwen, Zhilan Zhang, Xuhong Liu, Yiqin Cai, and Huinong Cai. 2013. "Cloning, Expression of the Major Capsid Protein Gene from Marine Algae Emiliania huxleyi Virus and the Possible Use in Detection of Virus Infection" Microbiology Research 4, no. 1: e5. https://doi.org/10.4081/mr.2013.e5

APA Style

Liu, J., Zhang, Z., Liu, X., Cai, Y., & Cai, H. (2013). Cloning, Expression of the Major Capsid Protein Gene from Marine Algae Emiliania huxleyi Virus and the Possible Use in Detection of Virus Infection. Microbiology Research, 4(1), e5. https://doi.org/10.4081/mr.2013.e5

Article Metrics

Back to TopTop