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Infectious Disease Reports
Volume 3
Issue 1
10.4081/idr.2011.e3
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Infectious Disease Reports is published by MDPI from Volume 12 Issue 3 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Article

Usefulness of the Polymerase Chain Reaction Dot-Blot Assay, Used with Ziehl-Neelsen Staining, for the Rapid and Conveni­ent Diagnosis of Pulmonary Tuberculosis in Human Immuno­deficiency Virus-Seropositive and -Seronegative Individuals

by
Luciene Cardoso Scherer
1,3,*,
Rosa D. Sperhacke
2,
Maria L. R. Rossetti
2,3,
Antonio Ruffino-Netto
4 and
Afrânio L. Kritski
5
1
Programa de Pós Graduação em Ciências Biológicas - Bioquímica, Universidade Federal do Rio Grande do Sul -UFRGS, Porto Alegre, RS, Brazil
2
Centro de Desenvolvimento de Ciência e Tecnologia- CDCT, Fundação Estadual de Produção e Pesquisa em Saúde-FEPPS/RS, Porto Alegre, RS, Brazil
3
Universidade Luterana do Brasil-ULBRA, Canoas, RS, Brazil
4
Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil
5
Unidade de Pesquisa em Tuberculose, Programa Acadêmico de TB, Hospital Universitário Clementino Fraga Filho, UPT, HUCFF, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, Brazil
*
Author to whom correspondence should be addressed.
Infect. Dis. Rep. 2011, 3(1), e3; https://doi.org/10.4081/idr.2011.e3
Submission received: 18 June 2010 / Revised: 22 January 2011 / Accepted: 16 March 2011 / Published: 24 March 2011

Abstract

There are scarce data regarding the value of molecular tests, when used in parallel with classical tools, for the diagnosis of tuberculosis (TB) under field conditions, especially in regions with a high burden of TB-human immunodeficiency virus (HIV) co-infection. We evaluated the usefulness of the polymerase chain reaction dot-blot assay (PCR) used in parallel with Ziehl-Neelsen staining (ZN) for pulmonary tuberculosis (PTB) diagnosis, in a TB-HIV reference hospital. All sputum samples from 277 patients were tested by ZN, culture, and PCR. Performances were assessed individually, in parallel, for HIV status, history of anti-TB treatment, and in different simulated TB prevalence rates. Overall, the PTB prevalence was 46% (128/277); in HIV-seropositive (HIV+) individuals, PTB prevalence was 54% (40/74); the ZN technique had a lower sensitivity (SE) in the HIV+ group than in the HIV-seronegative (HIV–) group (43% vs. 68%; Fisher test, P<0.05); and the SE of PCR was not affected by HIV status (Fisher test; P=0.46). ZN, in parallel with PCR, presented the following results: i) among all PTB suspects, SE of 90%, specificity (SP) of 84%, likelihood ratio (LR)+ of 5.65 and LR– of 0.12; ii) in HIV– subjects: SE of 92%, LR– of 0.10; iii) in not previously treated cases: SE of 90%, LR– of 0.11; iv) in TB, prevalence rates of 5-20%; negative predictive values (NPV) of 98-99%. ZN used in parallel with PCR showed an improvement in SE, LR–, and NPV, and may offer a novel approach in ruling out PTB cases, especially in not previously treated HIV– individuals, attended in hospitals in developing nations.
Keywords: in-house polymerase chain reaction; human immunodeficiency virus; tuberculosis in-house polymerase chain reaction; human immunodeficiency virus; tuberculosis

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MDPI and ACS Style

Scherer, L.C.; Sperhacke, R.D.; Rossetti, M.L.R.; Ruffino-Netto, A.; Kritski, A.L. Usefulness of the Polymerase Chain Reaction Dot-Blot Assay, Used with Ziehl-Neelsen Staining, for the Rapid and Conveni­ent Diagnosis of Pulmonary Tuberculosis in Human Immuno­deficiency Virus-Seropositive and -Seronegative Individuals. Infect. Dis. Rep. 2011, 3, e3. https://doi.org/10.4081/idr.2011.e3

AMA Style

Scherer LC, Sperhacke RD, Rossetti MLR, Ruffino-Netto A, Kritski AL. Usefulness of the Polymerase Chain Reaction Dot-Blot Assay, Used with Ziehl-Neelsen Staining, for the Rapid and Conveni­ent Diagnosis of Pulmonary Tuberculosis in Human Immuno­deficiency Virus-Seropositive and -Seronegative Individuals. Infectious Disease Reports. 2011; 3(1):e3. https://doi.org/10.4081/idr.2011.e3

Chicago/Turabian Style

Scherer, Luciene Cardoso, Rosa D. Sperhacke, Maria L. R. Rossetti, Antonio Ruffino-Netto, and Afrânio L. Kritski. 2011. "Usefulness of the Polymerase Chain Reaction Dot-Blot Assay, Used with Ziehl-Neelsen Staining, for the Rapid and Conveni­ent Diagnosis of Pulmonary Tuberculosis in Human Immuno­deficiency Virus-Seropositive and -Seronegative Individuals" Infectious Disease Reports 3, no. 1: e3. https://doi.org/10.4081/idr.2011.e3

APA Style

Scherer, L. C., Sperhacke, R. D., Rossetti, M. L. R., Ruffino-Netto, A., & Kritski, A. L. (2011). Usefulness of the Polymerase Chain Reaction Dot-Blot Assay, Used with Ziehl-Neelsen Staining, for the Rapid and Conveni­ent Diagnosis of Pulmonary Tuberculosis in Human Immuno­deficiency Virus-Seropositive and -Seronegative Individuals. Infectious Disease Reports, 3(1), e3. https://doi.org/10.4081/idr.2011.e3

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