Effect of Weekend Alcohol Consumption on Hepatic Antioxidant Enzyme Activity: Role of Concentration and Gender

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The authors evaluate the effect of binge alcohol consumption on hepatic antioxidant levels in mice. They found that 2-day binge drinking resulted in elevations of antioxidant levels, but not enough to counteract the harmful effects of alcohol on the liver.

• Was the study sufficiently powered to make comparisons and calculations? Can the authors include the power calculation performed to derive number of mice in each subset?
• What are the authors thoughts on the clinical implications of this work particularly since this work cannot be reproduced in human subjects in a clinical trial form? Do the authors have plans to expand on this work?
• The authors could consider rephrasing weekend to binge alcohol consumption as this seems to be the main area of focus
• Could the authors clarify the difference between "While men generally consume more alcohol, women have a higher rate of alcohol consumption"? (Page 2 lines 45-46) Do the authors mean total amount of alcohol versus number of days with alcohol consumption?
• Are there any differences between the different antioxidants in terms of function and importance?
• At least half of the reference list is comprised of self-citations. Have the authors considered broadening the scope of literature review to include studies outside their group?
• Page 7, section 3.6 line 261, consider changing to CAT for consistency
• What was the software used to generate figures?

Comments on the Quality of English Language

This can be improved through more succinct statements.

Author Response

The authors evaluate the effect of binge alcohol consumption on hepatic antioxidant levels in mice. They found that 2-day binge drinking resulted in elevations of antioxidant levels, but not enough to counteract the harmful effects of alcohol on the liver.

• Was the study sufficiently powered to make comparisons and calculations? Can the authors include the power calculation performed to derive number of mice in each subset?

Answer: Thank you for your comment, we appreciate it. As mentioned in the article, the statistics were performed between groups. Regarding the number of rats used in each group, the former is the one recommended by our research and cycle committees for the use of the smallest number of experimental organisms, it being important that the results are performed correctly, as was the case in our case.

 

• What are the authors thoughts on the clinical implications of this work particularly since this work cannot be reproduced in human subjects in a clinical trial form? Do the authors have plans to expand on this work?

Answer: Thank you very much for your comment. The number of people who consume alcohol over the weekend is significant, and the majority of the studies reported are related to the more social aspects. The clinical implication is that the weekend consumption of alcohol does indeed cause liver damage at the physiological and morphological levels and alteration of the profile of antioxidant enzymes. Even at the level of the oral cavity there is damage from this type of alcohol consumption. This manuscript is a continuation of several previously reported studies on the damage caused by consumption of alcohol over the weekend.

 

• The authors could consider rephrasing weekend to binge alcohol consumption as this seems to be the main area of focus

Answer: Thank you for your comment. Weekend alcohol consumption in the experimental groups was ad libitum, using a 40% concentration to compare it with the consumption of spirits and a 5% concentration to compare it with the consumption of beer. This model has already been published previously, thank you for your comment.

 

• Could the authors clarify the difference between "While men generally consume more alcohol, women have a higher rate of alcohol consumption"? (Page 2 lines 45-46) Do the authors mean total amount of alcohol versus number of days with alcohol consumption?

Answer: Thank you for the comment. This point was clarified in the article as follows:

It was also found that the main consumers of weekend alcohol are men; on the other hand, there are more and more women who consume weekend alcohol and are prone to liver damage due to alcohol.

 

 

 

 

 

 

• Are there any differences between the different antioxidants in terms of function and importance?

Answer: We consider the antioxidant processes that occur in the hepatocyte as a whole. The various antioxidant enzymes are interconnected with each other to provide a total response in defense of the various organelles. There are enzymes that are studied or reported to a greater extent than others, such as SOD, but all are important in relation to cytoprotection. This is made clear in the book.

 

Oxidative Stress and Chronic Degenerative Diseases - A Role for Antioxidants. ISBN978-953-51-1123-8. Doi: 10.5772/45722

 

• At least half of the reference list is comprised of self-citations. Have the authors considered broadening the scope of literature review to include studies outside their group?

Answer: Thank you for your suggestion. More quotes related to the topic from other authors have been added. As an explanation, the quotes from our research group are for continuity on the topic of liver damage due to alcohol, which we have been working on for years. Thank you.

 

• Page 7, section 3.6 line 261, consider changing to CAT for consistency

Answer: The change has already been made. Thanks for the observation.

 

 

• What was the software used to generate figures?

Answer: The program that was used was added to each figure. Thanks.

 

Reviewer 2 Report

Comments and Suggestions for Authors

The study focuses on a rather unconventional, but important aspect of studying the effects of alcohol on the liver - weekend consumption. A number of researchers have found that drinking alcohol on weekends produces an acute effect that is more pronounced in women. Thus, the peer-reviewed article is devoted to a rather important topic.

The authors formed 6 experimental groups of animals and studied the effect of alcohol consumption in different modes on Catalase enzyme activity ,superoxide dismutase activity, Glutathione Reductase Activity, Glutathione Peroxidase Activity  and other indicators.

The authors showed that alcohol consumption in all studied modes causes significant changes in the studied parameters, and they also established gender-specific features of these changes. These data are undoubtedly new and very interesting.

However, in my opinion, the work lacks data on classic markers characterizing the effect of alcohol on the liver: ALT, AST, total protein, albumin and others.

In addition, an increase in body weight implies a change in the morphology of organs, and it can be safely assumed that the structure of the liver will also change. A morphological study of this organ would be a good addition to the materials of the article.

Author Response

The study focuses on a rather unconventional, but important aspect of studying the effects of alcohol on the liver - weekend consumption. A number of researchers have found that drinking alcohol on weekends produces an acute effect that is more pronounced in women. Thus, the peer-reviewed article is devoted to a rather important topic.

Answer: Thanks for the comment.

 

The authors formed 6 experimental groups of animals and studied the effect of alcohol consumption in different modes on Catalase enzyme activity ,superoxide dismutase activity, Glutathione Reductase Activity, Glutathione Peroxidase Activity  and other indicators.

Answer: Thanks for the comment.

 

The authors showed that alcohol consumption in all studied modes causes significant changes in the studied parameters, and they also established gender-specific features of these changes. These data are undoubtedly new and very interesting.

Answer: Thanks for the comment.

 

However, in my opinion, the work lacks data on classic markers characterizing the effect of alcohol on the liver: ALT, AST, total protein, albumin and others.

Answer: Thank you for the comment. This manuscript is a continuation of several reports we have made on the damage caused by weekend alcohol consumption. I add the previously published articles on this experimental model:

1. Morales-González, J.A.; Sernas-Morales, M.L.; Morales-González, Á.; González-López, L.L.; Madrigal-Santillán, E.O.; Vargas-Mendoza, N.; Fregoso-Aguilar, T.A.; Anguiano-Robledo, L.; Madrigal-Bujaidar, E.; Álvarez-González, I.; et al. Morphological and biochemical effects of weekend alcohol consumption in rats: Role of concentration and gender. World J. Hepatol.2018, 10, 297–307. 
2. González-López, L.L.; Morales-González, Á.; Sosa-Gómez, A.; Madrigal-Santillán, E.O.; Anguiano-Robledo, L.; Madrigal-Bujaidar, E.; Álvarez-González, I.; Delgado-Olivares, L.; Valadez-Vega, C.; Esquivel-Chirino, C.; et al. Damage to Oral Mucosae Induced by Weekend Alcohol Consumption: The Role of Gender and Alcohol Concentration.  Sci.2022, 12, 3464. https://doi.org/10.3390/app12073464.

 

In addition, an increase in body weight implies a change in the morphology of organs, and it can be safely assumed that the structure of the liver will also change. A morphological study of this organ would be a good addition to the materials of the article.

Answer: Thank you for the comment. We previously published an article with this experimental model where the morphological study was carried out, which was the following:

 

1. Morales-González, J.A.; Sernas-Morales, M.L.; Morales-González, Á.; González-López, L.L.; Madrigal-Santillán, E.O.; Vargas-Mendoza, N.; Fregoso-Aguilar, T.A.; Anguiano-Robledo, L.; Madrigal-Bujaidar, E.; Álvarez-González, I.; et al. Morphological and biochemical effects of weekend alcohol consumption in rats: Role of concentration and gender. World J. Hepatol.2018, 10, 297–307. 

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

 the authors took into account all of my comments