Highly Effective Non-Viral Antitumor Gene Therapy System Comprised of Biocompatible Small Plasmid Complex Particles Consisting of pDNA, Anionic Polysaccharide, and Fully Deprotected Linear Polyethylenimine
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials and Mice
2.2. ζ-Potential and Size Measurement
2.3. In Vitro Transfection
2.3.1. Preparation of pDNA Complex for in Vitro Transfection
2.3.2. In Vitro Transfection
2.4. In Vivo Imaging
2.4.1. Preparation of Plasmid Complex for in Vivo Imaging
2.4.2. Image Acquisition
2.5. Treatment of Cancer
2.5.1. Preparation of Plasmid Complex for in Vivo Transfection
2.5.2. Evaluation of the Therapeutic Effect of the pDNA Complexes
3. Results and Discussion
3.1. Effect of Mixing Ratio of DNA and PEI “Max”
3.2. Effect of Dextran Addition on Rehydration Property
3.3. Effect of Mixing Ratio of HA (or CS)
3.4. Effect of Salt Strength and pH of the Rehydrating Solution
3.5. Effect of Salt Strength and pH of the Complex Preparation Conditions
3.6. In Vivo Imaging
3.7. Antitumor Efficacy of the Small pDNA/(PEI “Max”)/HA (or CS) Ternary Complex in the Tumor Model Mice
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Koyama, Y.; Sugiura, K.; Yoshihara, C.; Inaba, T.; Ito, T. Highly Effective Non-Viral Antitumor Gene Therapy System Comprised of Biocompatible Small Plasmid Complex Particles Consisting of pDNA, Anionic Polysaccharide, and Fully Deprotected Linear Polyethylenimine. Pharmaceutics 2015, 7, 152-164. https://doi.org/10.3390/pharmaceutics7030152
Koyama Y, Sugiura K, Yoshihara C, Inaba T, Ito T. Highly Effective Non-Viral Antitumor Gene Therapy System Comprised of Biocompatible Small Plasmid Complex Particles Consisting of pDNA, Anionic Polysaccharide, and Fully Deprotected Linear Polyethylenimine. Pharmaceutics. 2015; 7(3):152-164. https://doi.org/10.3390/pharmaceutics7030152
Chicago/Turabian StyleKoyama, Yoshiyuki, Kikuya Sugiura, Chieko Yoshihara, Toshio Inaba, and Tomoko Ito. 2015. "Highly Effective Non-Viral Antitumor Gene Therapy System Comprised of Biocompatible Small Plasmid Complex Particles Consisting of pDNA, Anionic Polysaccharide, and Fully Deprotected Linear Polyethylenimine" Pharmaceutics 7, no. 3: 152-164. https://doi.org/10.3390/pharmaceutics7030152
APA StyleKoyama, Y., Sugiura, K., Yoshihara, C., Inaba, T., & Ito, T. (2015). Highly Effective Non-Viral Antitumor Gene Therapy System Comprised of Biocompatible Small Plasmid Complex Particles Consisting of pDNA, Anionic Polysaccharide, and Fully Deprotected Linear Polyethylenimine. Pharmaceutics, 7(3), 152-164. https://doi.org/10.3390/pharmaceutics7030152