Composition Influence on Pulmonary Delivery of Rifampicin Liposomes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Liposomes.
RFP | P90H | P50 | Chol | OA | |
---|---|---|---|---|---|
mg/mL | mg/mL | mg/mL | mg/mL | mg/mL | |
PC30 | 10 | 15 | 15 | -- | -- |
PC30 Chol | 10 | 15 | 15 | 10 | -- |
PC30 Chol OA | 10 | 15 | 15 | 10 | 3 |
PC60 | 10 | 30 | 30 | -- | -- |
PC60 Chol | 10 | 30 | 30 | 10 | -- |
PC60 Chol OA | 10 | 30 | 30 | 10 | 6 |
Before dialysis | After dialysis | ||||||
---|---|---|---|---|---|---|---|
Size (nm) ± SD | PI | ZP (mV) ± SD | Size (nm) ± SD | PI | ZP (mV) ± SD | E (%) ± SD | |
PC30 | 106 ± 11 | 0.21 | −50 ± 2.8 | 101 ± 12 | 0.20 | −58 ± 0.4 | 67 ± 4 |
PC30 Chol | 128 ± 8 | 0.25 | −57 ± 3.1 | 118 ± 8 | 0.25 | −63 ± 2.9 | 64 ± 6 |
PC30 Chol OA | 143 ± 15 | 0.23 | −67 ± 3.3 | 153 ± 9 | 0.23 | −67 ± 3.7 | 60 ± 2 |
PC60 | 104 ± 3 | 0.14 | −54 ± 3.8 | 102±2 | 0.15 | −57 ± 1.3 | 76 ± 4 |
PC60 Chol | 112 ± 4 | 0.13 | −57 ± 6.6 | 108 ± 3 | 0.17 | −62 ± 1.2 | 74 ± 4 |
PC60 Chol OA | 79 ± 6 | 0.15 | −63 ± 7.4 | 85 ± 6 | 0.15 | −69 ± 3.3 | 69 ± 2 |
2.2. Rheological Studies
2.3. Nebulization Studies of Liposomes
NE% | FPD (µg) | FPF (%) | NER% | ||
---|---|---|---|---|---|
PC30 | Stage 1 | 35 ± 2.3 | 908 ± 23 | 9 ± 0.7 | 25 ± 0.7 |
Stage 2 | 5 ± 0.5 | ||||
Stage 3 | 4 ± 0.1 | ||||
PC30 Chol | Stage 1 | 48 ± 3.9 | 736 ± 12 | 7 ± 1.3 | 25 ± 1.0 |
Stage 2 | 5 ± 0.8 | ||||
Stage 3 | 3 ± 0.2 | ||||
PC30 Chol OA | Stage 1 | 51 ± 2.9 | 108 ± 32 | 11 ± 0.8 | 49 ± 3.3 |
Stage 2 | 7 ± 0.2 | ||||
Stage 3 | 4 ± 0.1 | ||||
PC60 | Stage 1 | 41 ± 6.8 | 1300 ± 37 | 13 ± 0.9 | 45 ± 3.1 |
Stage 2 | 10 ± 0.6 | ||||
Stage 3 | 3 ± 0.1 | ||||
PC60 Chol | Stage 1 | 54 ± 3.7 | 1260 ± 29 | 13 ± 1.2 | 49 ± 7.9 |
Stage 2 | 9 ± 0.2 | ||||
Stage 3 | 3 ± 0.1 | ||||
PC60 Chol OA | Stage 1 | 64 ± 2.3 | 1685 ± 35 | 17 ± 1.7 | 55 ± 1.4 |
Stage 2 | 12 ± 0.7 | ||||
Stage 3 | 5 ± 0.1 |
2.4. Cell Viability and Probe Uptake Studies
3. Experimental Section
3.1. Materials
3.2. Vesicle Preparation
3.3. Vesicle Characterization
3.4. Rheological Studies
3.5. Nebulization Studies of Liposomes
3.6. Cell Cultures
3.7. Cell Viability Studies (MTT Assay)
3.8. Cellular Uptake of Rho-PE Labeled CF-Loaded Vesicles
3.9. Statistical Analysis of Data
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Manca, M.L.; Sinico, C.; Maccioni, A.M.; Diez, O.; Fadda, A.M.; Manconi, M. Composition Influence on Pulmonary Delivery of Rifampicin Liposomes. Pharmaceutics 2012, 4, 590-606. https://doi.org/10.3390/pharmaceutics4040590
Manca ML, Sinico C, Maccioni AM, Diez O, Fadda AM, Manconi M. Composition Influence on Pulmonary Delivery of Rifampicin Liposomes. Pharmaceutics. 2012; 4(4):590-606. https://doi.org/10.3390/pharmaceutics4040590
Chicago/Turabian StyleManca, Maria Letizia, Chiara Sinico, Anna Maria Maccioni, Octavio Diez, Anna Maria Fadda, and Maria Manconi. 2012. "Composition Influence on Pulmonary Delivery of Rifampicin Liposomes" Pharmaceutics 4, no. 4: 590-606. https://doi.org/10.3390/pharmaceutics4040590