Useful Extend-release Chitosan Tablets with High Antioxidant Activity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Scavenging activity of LMW chitosans on DPPH radicals
2.3. Reducing power by using the TPAC test
2.4. ESR spectroscopy
2.5. Hydroxyl radical assay
2.6. Superoxide radical assay
2.7. Preparation of extended-release matrix tablet
2.8. Dissolution of TPH from tablets and scavenging activity of chitosans-TPH tablets on ABTS radicals
2.9. Statistics
3. Results and Discussion
3.1. Scavenging activity of LMW chitosans on DPPH radicals
Antioxidant | DPPH |
---|---|
IC50 (mg/mL) a | |
CS1 | 6 |
CS2 | 4.02 |
CS3 | 7.54 |
CS4 | >10 |
3.2. Reducing power of LMW chitosans by using the TPAC test
3.3. Radical scavenging potency of LMW chitosans by ESR spectroscopy
Conc. (g/mL) | Scavenging percentage (%) | ||||||||
---|---|---|---|---|---|---|---|---|---|
Hydroxyl radical | Superoxide radical | ||||||||
CS1 | CS2 | CS3 | CS4 | CS1 | CS2 | CS3 | CS4 | ||
100 | 53.7 ± 3.5 | 65.1 ± 4.5* | 50.5 ± 3.8 | 49.5 ± 4.7 | 39.7 ± 4.8 | 48.2 ± 5.8* | 33.5 ± 5.4 | 30.8 ± 5.2 | |
200 | 80.7 ± 4.5 | 92.1 ± 4.6* | 78.1 ± 4.6 | 75.1 ± 3.1 | 65.1 ± 4.6 | 80.1 ± 5.6* | 56.1 ± 5.8 | 50.1 ± 5.6 |
3.4. Release study of TPH and scavenging activity of chitosans-TPH tablet
3.5. Discussion
4. Conclusions
References and Notes
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Yasufuku, T.; Anraku, M.; Kondo, Y.; Hata, T.; Hirose, J.; Kobayashi, N.; Tomida, H. Useful Extend-release Chitosan Tablets with High Antioxidant Activity. Pharmaceutics 2010, 2, 245-257. https://doi.org/10.3390/pharmaceutics2020245
Yasufuku T, Anraku M, Kondo Y, Hata T, Hirose J, Kobayashi N, Tomida H. Useful Extend-release Chitosan Tablets with High Antioxidant Activity. Pharmaceutics. 2010; 2(2):245-257. https://doi.org/10.3390/pharmaceutics2020245
Chicago/Turabian StyleYasufuku, Taira, Makoto Anraku, Yuko Kondo, Toshiyuki Hata, Junzo Hirose, Nobuyuki Kobayashi, and Hisao Tomida. 2010. "Useful Extend-release Chitosan Tablets with High Antioxidant Activity" Pharmaceutics 2, no. 2: 245-257. https://doi.org/10.3390/pharmaceutics2020245