Comparative Study of the Potential Cell-Penetrating Peptide ∆M4 on Apoptosis Cell Signaling in A375 and A431 Cancer Cell Lines
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Cultures
2.2. Determination of Apoptosis Protein Expression
2.3. ROS Production Assay
2.4. Determination of Antioxidant Activity
2.5. Western Blot Analysis
2.6. Caspase 3/7 Assay
2.7. Statistical Analysis
3. Results and Discussion
3.1. Expression of Apoptotic Proteins by ∆M4 Treatment
3.2. ∆M4 Increases Intracellular ROS Production
3.3. ∆M4 Lowers the Cu/ZnSOD Activity in Melanoma and Epidermoid Carcinoma Cells
3.4. ∆M4 Decreases Catalase Activity in A375 Whereas It Has No Significant Effect in A431
3.5. ∆M4 Change Expression of the Stress–Response Transcription Factors in A375 and A431 Cell Lines
3.6. ∆M4 Induces Activation of Effector Caspases 3/7
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fandiño-Devia, E.; Brankiewicz, A.; Santa-González, G.A.; Guevara-Lora, I.; Manrique-Moreno, M. Comparative Study of the Potential Cell-Penetrating Peptide ∆M4 on Apoptosis Cell Signaling in A375 and A431 Cancer Cell Lines. Pharmaceutics 2024, 16, 775. https://doi.org/10.3390/pharmaceutics16060775
Fandiño-Devia E, Brankiewicz A, Santa-González GA, Guevara-Lora I, Manrique-Moreno M. Comparative Study of the Potential Cell-Penetrating Peptide ∆M4 on Apoptosis Cell Signaling in A375 and A431 Cancer Cell Lines. Pharmaceutics. 2024; 16(6):775. https://doi.org/10.3390/pharmaceutics16060775
Chicago/Turabian StyleFandiño-Devia, Estefanía, Aleksandra Brankiewicz, Gloria A. Santa-González, Ibeth Guevara-Lora, and Marcela Manrique-Moreno. 2024. "Comparative Study of the Potential Cell-Penetrating Peptide ∆M4 on Apoptosis Cell Signaling in A375 and A431 Cancer Cell Lines" Pharmaceutics 16, no. 6: 775. https://doi.org/10.3390/pharmaceutics16060775
APA StyleFandiño-Devia, E., Brankiewicz, A., Santa-González, G. A., Guevara-Lora, I., & Manrique-Moreno, M. (2024). Comparative Study of the Potential Cell-Penetrating Peptide ∆M4 on Apoptosis Cell Signaling in A375 and A431 Cancer Cell Lines. Pharmaceutics, 16(6), 775. https://doi.org/10.3390/pharmaceutics16060775