Next Article in Journal
Immunomodulatory Properties of Natural Extracts and Compounds Derived from Bidens pilosa L.: Literature Review
Next Article in Special Issue
Breast Milk Mesenchymal Stem Cells and/or Derived Exosomes Mitigated Adenine-Induced Nephropathy via Modulating Renal Autophagy and Fibrotic Signaling Pathways and Their Epigenetic Regulations
Previous Article in Journal
Recent Advances in the Microencapsulation of Essential Oils, Lipids, and Compound Lipids through Spray Drying: A Review
Previous Article in Special Issue
Wound-Healing Effects of Mesenchymal Stromal Cell Secretome in the Cornea and the Role of Exosomes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pharmaceutics
Volume 15
Issue 5
10.3390/pharmaceutics15051489
Review Report
pharmaceutics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Mesenchymal Stromal Cell Exosomes Mediate M2-like Macrophage Polarization through CD73/Ecto-5′-Nucleotidase Activity

Pharmaceutics 2023, 15(5), 1489; https://doi.org/10.3390/pharmaceutics15051489
by Kristeen Ye Wen Teo 1,2, Shipin Zhang 1,2, Jia Tong Loh 3, Ruenn Chai Lai 4, Hwee Weng Dennis Hey 1, Kong-Peng Lam 3, Sai Kiang Lim 4 and Wei Seong Toh 1,2,5,6,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Pharmaceutics 2023, 15(5), 1489; https://doi.org/10.3390/pharmaceutics15051489
Submission received: 17 April 2023 / Revised: 6 May 2023 / Accepted: 10 May 2023 / Published: 13 May 2023
(This article belongs to the Special Issue Mesenchymal Stem/Stromal Cell Extracellular Vesicles: From Active Principle to Next Generation Drug Delivery)

Round 1

Reviewer 1 Report

The manuscript by Teo et al. titled "Mesenchymal stromal cell exosomes mediate M2-like macrophage polarization through CD73/ecto-5' -nucleotidase activity" is an interesting work regarding MSC exosomes. The paper is well written and easy to follow because of the straight forward style and presentation. The figures are well put together and provide important and relevant information. Especially the methods figure and summary figure are extremely helpful for the reader. The manuscript would benefit from addressing the following minor points:

1) What is the rationale for using E1-MYC 16.3 ESC-derived MSCs for the studies? Why not other MSCs? How do these MSCs differ from MSCs that exist naturally in the body? Please explain in text and in the response.

2) Line 295, change "strongly indicate" to demonstrate or suggest.

3) Please include M2 macrophage characteristics observed in this study into Figure 7.

Author Response

Please see the attachment. 

Author Response File: Author Response.docx

Reviewer 2 Report

The manuscript studied the role of Mesenchymal stromal cell exosomes in M2-like macrophage polarization. The authors suggested that MSC exosomes promote M2-like macrophage polarization by catalyzing the production of adenosine, which then binds to adenosine receptors A2A and A2B to activate AKT/ERK-dependent signaling pathways. Also, polarization of M2-like macrophage by MSC exosomes was abolished in the presence of inhibitors of CD73 activity, adenosine receptors A2A and A2B, and AKT/ERK phosphorylation.

My comments

- In figure 6a: total ERK bands are partially cropped, and in p-AKT band for M0+Exo+wortmannin the background is different, please comment

-The inflammatory cytokines expression assessed by PCR should be confirmed by protein measurement by ELISA 

The manuscript needs revision for minor grammar and language mistakes

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 2 Report

The authors addressed my comments

English language needs minor revision

Back to TopTop