Cinnamic Acid Attenuates Peripheral and Hypothalamic Inflammation in High-Fat Diet-Induced Obese Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Computational Biology Experiment
2.1.1. Data Preparation
2.1.2. Active Component Prediction
2.1.3. Molecular Docking
2.2. Animal Experiment
2.2.1. Chemicals and Reagents
2.2.2. Experimental Design
2.2.3. Metabolic Phenotype Measurements
2.2.4. Biochemical Assays
2.2.5. Serum TNF-α and IL-6 Protein Levels
2.2.6. Preparation of Stromal Vascular Cells (SVCs)
2.2.7. Cell Staining and Fluorescence Activated Cell Sorting (FACS)
2.2.8. Histological Analysis of the Liver and Epididymal Fat Pad
2.2.9. Brain Tissue Preparation and IHC Staining
2.2.10. Quantitation of Activated Microglia and Astrocytes
2.2.11. Quantitative Reverse Transcription-PCR (qRT-PCR)
2.2.12. Statistical Analysis
3. Results
3.1. ADME Predictions
3.2. Molecular Docking
3.3. Metabolic Phenotypes
3.4. Glucose Metabolism and Insulin Resistance
3.5. Lipid Metabolism
3.6. Toxicity Biochemical Parameters
3.7. Expression of Inflammatory Cytokines
3.8. Inflammatory Monocytes
3.9. Fat Deposition and Macrophage Infiltration in Liver and Adipose Tissue
3.10. Adipose Tissue Macrophages (ATMs)
3.11. Inflammation in the Hypothalamus
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Lee, A.G.; Kang, S.; Im, S.; Pak, Y.K. Cinnamic Acid Attenuates Peripheral and Hypothalamic Inflammation in High-Fat Diet-Induced Obese Mice. Pharmaceutics 2022, 14, 1675. https://doi.org/10.3390/pharmaceutics14081675
Lee AG, Kang S, Im S, Pak YK. Cinnamic Acid Attenuates Peripheral and Hypothalamic Inflammation in High-Fat Diet-Induced Obese Mice. Pharmaceutics. 2022; 14(8):1675. https://doi.org/10.3390/pharmaceutics14081675
Chicago/Turabian StyleLee, Aden Geonhee, Sora Kang, Suyeol Im, and Youngmi Kim Pak. 2022. "Cinnamic Acid Attenuates Peripheral and Hypothalamic Inflammation in High-Fat Diet-Induced Obese Mice" Pharmaceutics 14, no. 8: 1675. https://doi.org/10.3390/pharmaceutics14081675
APA StyleLee, A. G., Kang, S., Im, S., & Pak, Y. K. (2022). Cinnamic Acid Attenuates Peripheral and Hypothalamic Inflammation in High-Fat Diet-Induced Obese Mice. Pharmaceutics, 14(8), 1675. https://doi.org/10.3390/pharmaceutics14081675