Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering
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Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 11031, Taiwan
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Department of Orthopedics, Taipei Medical University Hospital, Taipei 11031, Taiwan
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Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
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Orthopedics Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan
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Graduate Institute of Materials Science and Engineering, National Central University, Taoyuan 32001, Taiwan
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Department of Mechanical Engineering, National Central University, Taoyuan 32001, Taiwan
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School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan
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Department of Orthopedics, Taipei Medical University—Shuang Ho Hospital, New Taipei 11031, Taiwan
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School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
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Research Center of Biomedical Device, Taipei Medical University, Taipei 11031, Taiwan
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Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 11031, Taiwan
12
International PhD Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editors: Carlotta Pontremoli and Sonia Fiorilli
Pharmaceutics 2022, 14(5), 1078; https://doi.org/10.3390/pharmaceutics14051078
Received: 22 April 2022
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Revised: 14 May 2022
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Accepted: 16 May 2022
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Published: 17 May 2022
(This article belongs to the Special Issue Design of Mesoporous Materials for Biomedical Application)
Mg–Zn–Ca bulk metallic glass (BMG) is a promising orthopedic fixation implant because of its biodegradable and biocompatible properties. Structural supporting bone implants with osteoinduction properties for effective bone regeneration have been highly desired in recent years. Osteogenic growth peptide (OGP) can increase the proliferation and differentiation of mesenchymal stem cells and enhance the mineralization of osteoblast cells. However, the short half-life and non-specificity to target areas limit applications of OGP. Mesoporous silica nanoparticles (MSNs) as nanocarriers possess excellent properties, such as easy surface modification, superior targeting efficiency, and high loading capacity of drugs or proteins. Accordingly, we propose a system of combining the OGP-containing MSNs with Mg–Zn–Ca BMG materials to promote bone regeneration. In this work, we conjugated cysteine-containing OGP (cgOGP, 16 a.a.) to interior walls of channels in MSNs and maintained the dispersity of MSNs via PEGylation. An in vitro study showed that metal ions released from Mg–Zn–Ca BMG promoted cell proliferation and migration and elevated alkaline phosphatase (ALP) activity and mineralization. On treating cells with both BMG ion-containing Minimum Essential Medium Eagle-alpha modification (α-MEM) and OGP-conjugated MSNs, enhanced focal adhesion turnover and promoted differentiation were observed. Hematological analyses showed the biocompatible nature of this BMG/nanocomposite system. In addition, in vivo micro-computed tomographic and histological observations revealed that our system stimulated osteogenesis and new bone formation around the implant site.
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Keywords:
mesoporous silica nanoparticles; Mg–Zn–Ca bulk metallic glass; bone tissue engineering; osteogenic growth peptide; osteogenic differentiation; osteoinduction; osteoconduction; osseointegration
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MDPI and ACS Style
Chu, Y.S.; Wong, P.-C.; Jang, J.S.-C.; Chen, C.-H.; Wu, S.-H. Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering. Pharmaceutics 2022, 14, 1078. https://doi.org/10.3390/pharmaceutics14051078
AMA Style
Chu YS, Wong P-C, Jang JS-C, Chen C-H, Wu S-H. Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering. Pharmaceutics. 2022; 14(5):1078. https://doi.org/10.3390/pharmaceutics14051078
Chicago/Turabian StyleChu, Yun S., Pei-Chun Wong, Jason S.-C. Jang, Chih-Hwa Chen, and Si-Han Wu. 2022. "Combining Mg–Zn–Ca Bulk Metallic Glass with a Mesoporous Silica Nanocomposite for Bone Tissue Engineering" Pharmaceutics 14, no. 5: 1078. https://doi.org/10.3390/pharmaceutics14051078
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