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Article

Ethosomes and Transethosomes as Cutaneous Delivery Systems for Quercetin: A Preliminary Study on Melanoma Cells

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Department of Neuroscience and Rehabilitation, University of Ferrara, I-44121 Ferrara, Italy
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Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, I-44121-Ferrara, Italy
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Department of Life Sciences and Biotechnology, University of Ferrara, I-44121 Ferrara, Italy
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Department of Environmental and Prevention Sciences, University of Ferrara, I-44121 Ferrara, Italy
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Plants for Human Health Institute, Department of Animal Science, NC Research Campus Kannapolis, NC State University, Kannapolis, NC 28081, USA
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Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Donatella Paolino
Pharmaceutics 2022, 14(5), 1038; https://doi.org/10.3390/pharmaceutics14051038
Received: 14 April 2022 / Revised: 8 May 2022 / Accepted: 9 May 2022 / Published: 11 May 2022
(This article belongs to the Special Issue Nanotechnology-Enabled Strategies to Enhance Topical Bioavailability)
The present study is aimed to design ethosomes and transethosomes for topical administration of quercetin. To overcome quercetin low bioavailability, scarce solubility and poor permeability that hamper its pharmaceutical use, the drug was loaded in ethosomes and transethosomes based on different concentrations of phosphatidylcholine. Vesicle morphology was studied by cryogenic transmission electron microscopy, while size distribution and quercetin entrapment capacity were evaluated up to 3 months, respectively, by photon correlation spectroscopy and high-performance liquid chromatography. The antioxidant property was studied by photochemiluminescence test. Quercetin release and permeation was investigated in vitro, using Franz cells associated to different membranes. In vitro assays were conducted on human keratinocytes and melanoma cells to study the behavior of quercetin-loaded nano-vesicular forms with respect to cell migration and proliferation. The results evidenced that both phosphatidylcholine concentration and quercetin affected the vesicle size. Quercetin entrapment capacity, antioxidant activity and size stability were controlled using transethosomes produced by the highest amount of phosphatidylcholine. In vitro permeation studies revealed an enhancement of quercetin permeation in the case of transethosomes with respect to ethosomes. Notably, scratch wound and migration assays suggested the potential of quercetin loaded-transethosomes as adjuvant strategy for skin conditions. View Full-Text
Keywords: quercetin; ethosome; transethosome; in vitro release test; in vitro permeation test; Franz cell; wound healing; migration assay quercetin; ethosome; transethosome; in vitro release test; in vitro permeation test; Franz cell; wound healing; migration assay
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MDPI and ACS Style

Ferrara, F.; Benedusi, M.; Sguizzato, M.; Cortesi, R.; Baldisserotto, A.; Buzzi, R.; Valacchi, G.; Esposito, E. Ethosomes and Transethosomes as Cutaneous Delivery Systems for Quercetin: A Preliminary Study on Melanoma Cells. Pharmaceutics 2022, 14, 1038. https://doi.org/10.3390/pharmaceutics14051038

AMA Style

Ferrara F, Benedusi M, Sguizzato M, Cortesi R, Baldisserotto A, Buzzi R, Valacchi G, Esposito E. Ethosomes and Transethosomes as Cutaneous Delivery Systems for Quercetin: A Preliminary Study on Melanoma Cells. Pharmaceutics. 2022; 14(5):1038. https://doi.org/10.3390/pharmaceutics14051038

Chicago/Turabian Style

Ferrara, Francesca, Mascia Benedusi, Maddalena Sguizzato, Rita Cortesi, Anna Baldisserotto, Raissa Buzzi, Giuseppe Valacchi, and Elisabetta Esposito. 2022. "Ethosomes and Transethosomes as Cutaneous Delivery Systems for Quercetin: A Preliminary Study on Melanoma Cells" Pharmaceutics 14, no. 5: 1038. https://doi.org/10.3390/pharmaceutics14051038

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