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Article

Thermoresponsive Hydrogel Containing Viscum album Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity

1
Laboratório Multidisciplinar em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil
2
Hiscia Institute, Society for Cancer Research, 4144 Arlesheim, Switzerland
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Laboratório de Desenvolvimento Galênico, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil
4
Laboratório de Alimentos, Instituto Federal de Educacão, Ciência e Tecnologia do Rio de Janeiro, Rio de Janeiro 20270-021, RJ, Brazil
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Laboratório de Diagnóstico Molecular e Hematologia, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil
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LaRBio Carlos Chagas Filho, Biophysics Institute, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil
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Laboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niteroi 24241-000, RJ, Brazil
8
Institute of Complementary and Integrative Medicine, University of Bern, 3012 Bern, Switzerland
9
Institute for Integrative Medicine, University of Witten/Herdecke, 58455 Herdecke, Germany
*
Authors to whom correspondence should be addressed.
Academic Editors: Javier Garcia-Pardo, Maria Camilla Bergonzi and Charles M. Heard
Pharmaceutics 2022, 14(1), 37; https://doi.org/10.3390/pharmaceutics14010037
Received: 25 November 2021 / Revised: 16 December 2021 / Accepted: 21 December 2021 / Published: 24 December 2021
Viscum album L. (Santalaceae), also known as European mistletoe, is a semi-parasitic plant that grows on different host trees. Our group recently demonstrated the antitumoral activity of ethanolic V. album extracts in vitro, depending on the dose and the host tree, V. album ssp abietis from Abies alba being the most active extract. The goal of this work focused on the development of a new topical formulation containing V. album extracts, evaluation of in vitro toxicity and ex vivo skin permeation assays. The Poloxamer 407 hydrogel containing 5% of dry (VA_DEH) or aqueous (VA_AEH) extract presented dermal compatible pH and microbiological stability for 180 days. The hydrogels flow curve presented a non-linear relation, characteristic of non-Newtonian fluids, and the mean viscosity for the VA_DEH and VA_AEH was 372.5 ± 7.78 and 331.0 ± 2.83 Pa.s, respectively, being statistically different (Welch’s t test; p < 0.01). Additionally, WST-1 in vitro assays revealed a dose-dependent toxicity for both formulations and VA_DEH presented a higher activity than the VA_AEH. The promising cytotoxic potential of VA_DEH lead to the ex vivo skin permeation assay with 2.73 ± 0.19 µg/cm2 of chlorogenic acid, which permeated at 8 h, showing a transdermal potential. These in vitro results support the idea that VA_DEH is a novel promising candidate for mistletoe therapy. Therefore, further in vivo and pre-clinical experiments should be performed to evaluate the safety and efficacy of this new dermic delivery system. View Full-Text
Keywords: mistletoe; Viscum album; thermoresponsive hydrogel; rheology; stability; toxicity test; permeation mistletoe; Viscum album; thermoresponsive hydrogel; rheology; stability; toxicity test; permeation
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MDPI and ACS Style

Batista, J.V.D.C.; Matos, A.P.S.; Oliveria, A.P.; Ricci Júnior, E.; Freitas, Z.M.; Oliveira, C.A.; Toma, H.K.; Capella, M.A.M.; Rocha, L.M.; Weissenstein, U.; Baumgartner, S.; Holandino, C. Thermoresponsive Hydrogel Containing Viscum album Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity. Pharmaceutics 2022, 14, 37. https://doi.org/10.3390/pharmaceutics14010037

AMA Style

Batista JVDC, Matos APS, Oliveria AP, Ricci Júnior E, Freitas ZM, Oliveira CA, Toma HK, Capella MAM, Rocha LM, Weissenstein U, Baumgartner S, Holandino C. Thermoresponsive Hydrogel Containing Viscum album Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity. Pharmaceutics. 2022; 14(1):37. https://doi.org/10.3390/pharmaceutics14010037

Chicago/Turabian Style

Batista, João V. D. C., Ana Paula S. Matos, Adriana P. Oliveria, Eduardo Ricci Júnior, Zaida M. Freitas, Catarina A. Oliveira, Helena K. Toma, Marcia A. M. Capella, Leandro M. Rocha, Ulrike Weissenstein, Stephan Baumgartner, and Carla Holandino. 2022. "Thermoresponsive Hydrogel Containing Viscum album Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity" Pharmaceutics 14, no. 1: 37. https://doi.org/10.3390/pharmaceutics14010037

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