Lipid Nanoparticle-Mediated Lymphatic Delivery of Immunostimulatory Nucleic Acids
1
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea
2
School of Systems Biomedical Science, Soongsil University, Seoul 06978, Korea
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Imran Saleem
Pharmaceutics 2021, 13(4), 490; https://doi.org/10.3390/pharmaceutics13040490
Received: 4 February 2021
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Revised: 22 March 2021
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Accepted: 30 March 2021
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Published: 3 April 2021
(This article belongs to the Special Issue Recent Approaches for Lymphatic Drug Delivery)
Lymphatic delivery of a vaccine can be achieved using a dendritic cell (DC)-targeted delivery system that can cause DC to migrate to lymph nodes upon activation by an adjuvant. Here, we designed a mannose-modified cationic lipid nanoparticle (M-NP) to deliver the nucleic acid adjuvant, polyinosinic:polycytidylic acid (PIC). PIC-loaded M-NP (PIC/M-NP) showed stable lipoplexes regardless of the ligand ratio and negligible cytotoxicity in bone marrow-derived DC. DC uptake of PIC/M-NP was demonstrated, and an increased mannose ligand ratio improved DC uptake efficiency. PIC/M-NP significantly promoted the maturation of bone marrow-derived DC, and local injection of PIC/M-NP to mice facilitated lymphatic delivery and activation (upon NP uptake) of DC. Our results support the potential of PIC/M-NP in delivering a nucleic acid adjuvant for the vaccination of antigens.
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Keywords:
nucleic acid adjuvant; lipid nanoparticle; lymphatic delivery; dendritic cell-targeted delivery
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MDPI and ACS Style
Kim, D.; Wu, Y.; Shim, G.; Oh, Y.-K. Lipid Nanoparticle-Mediated Lymphatic Delivery of Immunostimulatory Nucleic Acids. Pharmaceutics 2021, 13, 490. https://doi.org/10.3390/pharmaceutics13040490
AMA Style
Kim D, Wu Y, Shim G, Oh Y-K. Lipid Nanoparticle-Mediated Lymphatic Delivery of Immunostimulatory Nucleic Acids. Pharmaceutics. 2021; 13(4):490. https://doi.org/10.3390/pharmaceutics13040490
Chicago/Turabian StyleKim, Dongyoon, Yina Wu, Gayong Shim, and Yu-Kyoung Oh. 2021. "Lipid Nanoparticle-Mediated Lymphatic Delivery of Immunostimulatory Nucleic Acids" Pharmaceutics 13, no. 4: 490. https://doi.org/10.3390/pharmaceutics13040490
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