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Article

Low Blue Dose Photodynamic Therapy with Porphyrin-Iron Oxide Nanoparticles Complexes: In Vitro Study on Human Melanoma Cells

1
National Institute of Laser, Plasma and Radiation Physics, 409 Atomistilor Str., 077125 Magurele, Romania
2
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
3
Research Institute of the University of Bucharest, Earth, Environmental and Life Sciences, Section-ICUB, 050663 Bucharest, Romania
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Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh. Polizu Str., 011061 Bucharest, Romania
5
National Institute of Materials Physics, 405 A Atomistilor Str., 077125 Magurele, Romania
*
Authors to whom correspondence should be addressed.
Academic Editor: Maria Nowakowska
Pharmaceutics 2021, 13(12), 2130; https://doi.org/10.3390/pharmaceutics13122130
Received: 2 November 2021 / Revised: 1 December 2021 / Accepted: 3 December 2021 / Published: 10 December 2021
(This article belongs to the Special Issue Novel Anticancer Strategies (Volume II))
The purpose of this study was to investigate the effectiveness in photodynamic therapy of iron oxide nanoparticles (γ-Fe2O3 NPs), synthesized by laser pyrolysis technique, functionalized with 5,10,15,20-(Tetra-4-sulfonatophenyl) porphyrin tetraammonium (TPPS) on human cutaneous melanoma cells, after only 1 min blue light exposure. The efficiency of porphyrin loading on the iron oxide nanocarriers was estimated by using absorption and FTIR spectroscopy. The singlet oxygen yield was determined via transient characteristics of singlet oxygen phosphorescence at 1270 nm both for porphyrin functionalized nanoparticles and rose bengal used as standard. The irradiation was performed with a LED (405 nm, 1 mW/cm2) for 1 min after melanoma cells were treated with TPPS functionalized iron oxide nanoparticles (γ-Fe2O3 NPs_TPPS) and incubated for 24 h. Biological tests revealed a high anticancer effect of γ-Fe2O3 NPs_TPPS complexes indi-cated by the inhibition of tumor cell proliferation, reduction of cell adhesion, and induction of cell death through ROS generated by TPPS under light exposure. The biological assays were combined with the pharmacokinetic prediction of the porphyrin. View Full-Text
Keywords: photodynamic therapy PDT; porphyrin; iron oxide nanoparticles; melanoma photodynamic therapy PDT; porphyrin; iron oxide nanoparticles; melanoma
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MDPI and ACS Style

Nistorescu, S.; Udrea, A.-M.; Badea, M.A.; Lungu, I.; Boni, M.; Tozar, T.; Dumitrache, F.; Maraloiu, V.-A.; Popescu, R.G.; Fleaca, C.; Andronescu, E.; Dinischiotu, A.; Staicu, A.; Balas, M. Low Blue Dose Photodynamic Therapy with Porphyrin-Iron Oxide Nanoparticles Complexes: In Vitro Study on Human Melanoma Cells. Pharmaceutics 2021, 13, 2130. https://doi.org/10.3390/pharmaceutics13122130

AMA Style

Nistorescu S, Udrea A-M, Badea MA, Lungu I, Boni M, Tozar T, Dumitrache F, Maraloiu V-A, Popescu RG, Fleaca C, Andronescu E, Dinischiotu A, Staicu A, Balas M. Low Blue Dose Photodynamic Therapy with Porphyrin-Iron Oxide Nanoparticles Complexes: In Vitro Study on Human Melanoma Cells. Pharmaceutics. 2021; 13(12):2130. https://doi.org/10.3390/pharmaceutics13122130

Chicago/Turabian Style

Nistorescu, Simona, Ana-Maria Udrea, Madalina Andreea Badea, Iulia Lungu, Mihai Boni, Tatiana Tozar, Florian Dumitrache, Valentin-Adrian Maraloiu, Roua Gabriela Popescu, Claudiu Fleaca, Ecaterina Andronescu, Anca Dinischiotu, Angela Staicu, and Mihaela Balas. 2021. "Low Blue Dose Photodynamic Therapy with Porphyrin-Iron Oxide Nanoparticles Complexes: In Vitro Study on Human Melanoma Cells" Pharmaceutics 13, no. 12: 2130. https://doi.org/10.3390/pharmaceutics13122130

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