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Article

Angiotensin-(1–7) Peptide Hormone Reduces Inflammation and Pathogen Burden during Mycoplasma pneumoniae Infection in Mice

1
Department of Immunobiology, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA
2
Asthma and Airway Disease Research Center, Tucson, AZ 85724, USA
3
Departments of Chemistry and Biochemistry, College of Science, The University of Arizona, Tucson, AZ 85721, USA
4
Department of Physiology, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA
5
BIO5 Institute, The University of Arizona, Tucson, AZ 85719, USA
6
Department of Medicine, Division of Translational & Regenerative Medicine, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA
7
Departments of Pharmacology/Toxicology and Pharmaceutical Sciences, College of Pharmacy, The University of Arizona, Tucson, AZ 85724, USA
8
Department of Cellular and Molecular Medicine, College of Medicine, The University of Arizona, Tucson, AZ 85721, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Philip Chi Lip Kwok and Michael Yee Tak Chow
Pharmaceutics 2021, 13(10), 1614; https://doi.org/10.3390/pharmaceutics13101614
Received: 17 August 2021 / Revised: 30 September 2021 / Accepted: 1 October 2021 / Published: 4 October 2021
(This article belongs to the Special Issue Inhaled Treatment of Respiratory Infections)
The peptide hormone, angiotensin (Ang-(1–7)), produces anti-inflammatory and protective effects by inhibiting production and expression of many cytokines and adhesion molecules that are associated with a cytokine storm. While Ang-(1–7) has been shown to reduce inflammation and airway hyperreactivity in models of asthma, little is known about the effects of Ang-(1–7) during live respiratory infections. Our studies were developed to test if Ang-(1–7) is protective in the lung against overzealous immune responses during an infection with Mycoplasma pneumonia (Mp), a common respiratory pathogen known to provoke exacerbations in asthma and COPD patients. Wild type mice were treated with infectious Mp and a subset of was given either Ang-(1–7) or peptide-free vehicle via oropharyngeal delivery within 2 h of infection. Markers of inflammation in the lung were assessed within 24 h for each set of animals. During Mycoplasma infection, one high dose of Ang-(1–7) delivered to the lungs reduced neutrophilia and Muc5ac, as well as Tnf-α and chemokines (Cxcl1) associated with acute respiratory distress syndrome (ARDS). Despite decreased inflammation, Ang-(1-7)-treated mice also had significantly lower Mp burden in their lung tissue, indicating decreased airway colonization. Ang-(1–7) also had an impact on RAW 264.7 cells, a commonly used macrophage cell line, by dose-dependently inhibiting TNF-α production while promoting Mp killing. These new findings provide additional support to the protective role(s) of Ang1-7 in controlling inflammation, which we found to be highly protective against live Mp-induced lung inflammation. View Full-Text
Keywords: angiotensin-(1–7); Mycoplasma pneumoniae; asthma; inflammation; macrophages angiotensin-(1–7); Mycoplasma pneumoniae; asthma; inflammation; macrophages
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MDPI and ACS Style

Collins, K.L.; Younis, U.S.; Tanyaratsrisakul, S.; Polt, R.; Hay, M.; Mansour, H.M.; Ledford, J.G. Angiotensin-(1–7) Peptide Hormone Reduces Inflammation and Pathogen Burden during Mycoplasma pneumoniae Infection in Mice. Pharmaceutics 2021, 13, 1614. https://doi.org/10.3390/pharmaceutics13101614

AMA Style

Collins KL, Younis US, Tanyaratsrisakul S, Polt R, Hay M, Mansour HM, Ledford JG. Angiotensin-(1–7) Peptide Hormone Reduces Inflammation and Pathogen Burden during Mycoplasma pneumoniae Infection in Mice. Pharmaceutics. 2021; 13(10):1614. https://doi.org/10.3390/pharmaceutics13101614

Chicago/Turabian Style

Collins, Katie L., Usir S. Younis, Sasipa Tanyaratsrisakul, Robin Polt, Meredith Hay, Heidi M. Mansour, and Julie G. Ledford 2021. "Angiotensin-(1–7) Peptide Hormone Reduces Inflammation and Pathogen Burden during Mycoplasma pneumoniae Infection in Mice" Pharmaceutics 13, no. 10: 1614. https://doi.org/10.3390/pharmaceutics13101614

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