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Open AccessArticle

Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns

1
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
2
Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain
3
Nanoscience & Nanotechnology Institute (IN2UB), University of Barcelona, 08028 Barcelona, Spain
4
Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain
5
Nutrition and Food Safety Research Institute (INSA-UB), 08921 Santa Coloma de Gramenet, Spain
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Department of Biology, Healthcare and the Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, 27-31 Joan XXIII Ave., 08028 Barcelona, Spain
7
Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, 62209 Cuernavaca, Mexico
8
Biosanitary Institute of Granada (ibs.GRANADA), 18012 Granada, Spain
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2021, 13(1), 8; https://doi.org/10.3390/pharmaceutics13010008
Received: 26 November 2020 / Revised: 14 December 2020 / Accepted: 17 December 2020 / Published: 22 December 2020
(This article belongs to the Special Issue Nano-Micro Encapsulation of Drugs)
The main goal of this work is the study of the skin wound healing efficacy of an antioxidant cocktail consisting of vitamins A, D, E and the endogenous pineal hormone melatonin (MLT), with all of these loaded into a thermosensitive hydrogel delivery system. The resulting formulation was characterized by scanning electron microscopy. The antioxidant efficacy and microbiological activity against Gram positive and Gram negative strains were also assayed. The skin healing efficacy was tested using an in vivo model which included histological evaluation. Furthermore, atomic force microscopy was employed to evaluate the wound healing efficacy of rat skin burns through the determination of its elasticity at the nanoscale using force spectroscopy analysis. The resulting hydrogel exhibited sol state at low temperature and turned into a gel at 30 ± 0.2 °C. The hydrogel containing the antioxidant cocktail showed higher scavenging activity than the hydrogel containing vitamins or MLT, separately. The formulation showed optimal antimicrobial activity. It was comparable to a commercial reference. It was also evidenced that the hydrogel containing the antioxidant cocktail exhibited the strongest healing process in the skin burns of rats, similar to the assayed commercial reference containing silver sulfadiazine. Histological studies confirmed the observed results. Finally, atomic force microscopy demonstrated a similar distribution of Young’s modulus values between burned skin treated with the commercial reference and burned skin treated with hydrogel containing the antioxidant cocktail, and all these with healthy skin. The use of an antioxidant cocktail of vitamins and MLT might be a promising treatment for skin wounds for future clinical studies. View Full-Text
Keywords: vitamins; melatonin; antioxidant; skin; healing; ATF microscopy vitamins; melatonin; antioxidant; skin; healing; ATF microscopy
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MDPI and ACS Style

Soriano, J.L.; Calpena, A.C.; Rodríguez-Lagunas, M.J.; Domènech, Ò.; Bozal-de Febrer, N.; Garduño-Ramírez, M.L.; Clares, B. Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns. Pharmaceutics 2021, 13, 8. https://doi.org/10.3390/pharmaceutics13010008

AMA Style

Soriano JL, Calpena AC, Rodríguez-Lagunas MJ, Domènech Ò, Bozal-de Febrer N, Garduño-Ramírez ML, Clares B. Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns. Pharmaceutics. 2021; 13(1):8. https://doi.org/10.3390/pharmaceutics13010008

Chicago/Turabian Style

Soriano, José L.; Calpena, Ana C.; Rodríguez-Lagunas, María J.; Domènech, Òscar; Bozal-de Febrer, Nuria; Garduño-Ramírez, María L.; Clares, Beatriz. 2021. "Endogenous Antioxidant Cocktail Loaded Hydrogel for Topical Wound Healing of Burns" Pharmaceutics 13, no. 1: 8. https://doi.org/10.3390/pharmaceutics13010008

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