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Open AccessArticle

Nanoparticle-Delivered HIV Peptides to Dendritic Cells a Promising Approach to Generate a Therapeutic Vaccine

1
Section of Immunology, ImmunoBiology Molecular Laboratory, Spanish HIV HGM BioBank, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain
2
Department of. Química Orgánica, Facultad de Química, Universidad de Sevilla, 41012 Sevilla, Spain
3
Department of Chemistry & Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA
4
Institute for Chemical Research, CSIC–University of Sevilla, 41092 Sevilla, Spain
5
CIBERNED, Instituto de Salud Carlos III, 28031 Madrid, Spain
6
Unidad Asociada Neurodeath, Facultad de Medicina, Universidad de Castilla-La Mancha, 02006 Albacete, Spain
7
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III, 28034 Madrid, Spain
*
Author to whom correspondence should be addressed.
Pharmaceutics 2020, 12(7), 656; https://doi.org/10.3390/pharmaceutics12070656
Received: 13 May 2020 / Revised: 4 July 2020 / Accepted: 6 July 2020 / Published: 11 July 2020
(This article belongs to the Special Issue Cancer Nanomedicine—From the Bench to the Bedside)
Finding a functional cure for HIV-1 infection will markedly decrease the social and economic burden of this disease. In this work, we have taken advantage of the antigen presenting cell role of human dendritic cells (DCs) to try to induce an immune response to HIV-derived peptide delivered to DCs using two different polycationic nanoparticles: a G4 PAMAM dendrimer modified to a 70/30 ratio of hydroxyl groups/amines and a cyclodextrin derivative. We have studied peptide delivery using a fluorescence peptide and have studied the immune response generation by cytokine determination and flow cytometry. We have found a robust delivery of the antigenic peptide to DCs and activated dendritic cell-mediated peripheral blood mononuclear cells (PBMCs) proliferation using the mixed lymphocyte reaction. However, no expression of markers indicating activation of either B or T lymphocytes was observed. Moreover, the release of the pro-inflammatory cytokine TNF-α or IL-2 was only observed when DCs treated with either the dendrimer or the dendriplex containing the peptide. Antigenic peptide delivery to DCs is a promising approach to generate a vaccine against HIV-1 infection. However, more studies, including the simultaneous delivery of several antigenic peptides from different viral proteins, can markedly improve the immune response. View Full-Text
Keywords: HIV-1; DCs; polycationic nanoparticles; delivery; fluorescence peptides; cytokines; vaccine HIV-1; DCs; polycationic nanoparticles; delivery; fluorescence peptides; cytokines; vaccine
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MDPI and ACS Style

Martín-Moreno, A.; Jiménez Blanco, J.L.; Mosher, J.; Swanson, D.R.; García Fernández, J.M.; Sharma, A.; Ceña, V.; Muñoz-Fernández, M.A. Nanoparticle-Delivered HIV Peptides to Dendritic Cells a Promising Approach to Generate a Therapeutic Vaccine. Pharmaceutics 2020, 12, 656.

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