Next Article in Journal
A Mixed-Surface Polyamidoamine Dendrimer for In Vitro and In Vivo Delivery of Large Plasmids
Previous Article in Journal
Improvement in the Oral Bioavailability and Efficacy of New Ezetimibe Formulations—Comparative Study of a Solid Dispersion and Different Micellar Systems
Previous Article in Special Issue
Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration
Open AccessArticle

Nitric Oxide-Releasing S-Nitrosoglutathione-Conjugated Poly(Lactic-Co-Glycolic Acid) Nanoparticles for the Treatment of MRSA-Infected Cutaneous Wounds

1
College of Pharmacy, Pusan National University, Busan 46241, Korea
2
Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to the article.
Pharmaceutics 2020, 12(7), 618; https://doi.org/10.3390/pharmaceutics12070618
Received: 8 June 2020 / Revised: 25 June 2020 / Accepted: 30 June 2020 / Published: 2 July 2020
(This article belongs to the Special Issue Bioadhesive Systems for Topical Application)
S-nitrosoglutathione (GSNO) has emerged as a potent agent for the treatment of infected cutaneous wounds. However, fabrication of GSNO-containing nanoparticles has been challenging due to its high hydrophilicity and degradability. The present study aimed to fabricate nanoparticles using newly synthesized GSNO-conjugated poly(lactic-co-glycolic acid) (PLGA) (GSNO-PLGA; GPNPs). Since hydrophilic GSNO was covalently bound to hydrophobic PLGA, loss of GSNO during the nanoparticle fabrication process was minimized, resulting in sufficient loading efficiency (2.32% of GSNO, 0.07 μmol/mg of NO). Real-time NO release analysis revealed biphasic NO release by GPNPs, including initial burst release within 3 min and continuous controlled release for up to 11.27 h, due to the differential degradation rates of the –SNO groups located at the surface and inside of GPNPs. Since GPNPs could deliver NO more efficiently than GSNO in response to increased interaction with bacteria, the former showed enhanced antibacterial effects against methicillin-resistant Staphylococcus aureus (MRSA) at the same equivalent concentrations of NO. Finally, the facilitating effects of GPNPs on infected wound healing were demonstrated in MRSA-challenged full-thickness wound mouse model. Collectively, the results suggested GPNPs as an ideal nanoparticle formulation for the treatment of MRSA-infected cutaneous wounds. View Full-Text
Keywords: S-nitrosoglutathione (GSNO); poly(lactic-co-glycolic acid) (PLGA); nitric oxide; nitric oxide-releasing nanoparticles; GSNO-conjugated PLGA; methicillin-resistant Staphylococcus aureus (MRSA); infected wound healing S-nitrosoglutathione (GSNO); poly(lactic-co-glycolic acid) (PLGA); nitric oxide; nitric oxide-releasing nanoparticles; GSNO-conjugated PLGA; methicillin-resistant Staphylococcus aureus (MRSA); infected wound healing
Show Figures

Graphical abstract

MDPI and ACS Style

Lee, J.; Kwak, D.; Kim, H.; Kim, J.; Hlaing, S.P.; Hasan, N.; Cao, J.; Yoo, J.-W. Nitric Oxide-Releasing S-Nitrosoglutathione-Conjugated Poly(Lactic-Co-Glycolic Acid) Nanoparticles for the Treatment of MRSA-Infected Cutaneous Wounds. Pharmaceutics 2020, 12, 618.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop