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Open AccessArticle

Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy

by Wei Mao 1, Sol Lee 1, Ji Un Shin 1 and Hyuk Sang Yoo 1,2,*
1
Department of Biomedical Materials Engineering, Kangwon National University, Chuncheon 24341, Korea
2
Institute for Molecular Science and Fusion Technology, Kangwon National University, Chuncheon 24341, Korea
*
Author to whom correspondence should be addressed.
Pharmaceutics 2020, 12(3), 261; https://doi.org/10.3390/pharmaceutics12030261
Received: 13 January 2020 / Revised: 10 March 2020 / Accepted: 11 March 2020 / Published: 13 March 2020
Surface initiated atom transfer radical polymerization (SI-ATRP) documented a simple but efficient technique to grow a dense polymer layer on any surface. Gold nanoparticles (AuNPs) give a broad surface to immobilize sulfhyryl group-containing initiators for SI-ATRP; in addition, AuNPs are the major nanoparticulate carriers for delivery of anti-cancer therapeutics, since they are biocompatible and bioinert. In this work, AuNPs with a disulfide initiator were polymerized with sulfoethyl methacrylate by SI-ATRP to decorate the particles with anionic corona, and branched polyethyeleneimine (PEI) and siRNA were sequentially layered onto the anionic corona of AuNP by electrostatic interaction. The in vitro anti-cancer effect confirmed that AuNP with anionic corona showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study of tumor-bearing nude mice revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size in comparison to those with free siRNA for 4 weeks. Furthermore, histological examination and gene expression study revealed that the decorated AuNP significantly suppressed c-Myc expression. Thus, we envision that the layer-by-layer assembly on the anionic brushes can be potentially used to incorporate nucleic acids onto metallic particles with high transfection efficiency. View Full-Text
Keywords: surface-initiated atom transfer radical polymerization; gold nanoparticle; c-Myc siRNA; anti-cancer therapy surface-initiated atom transfer radical polymerization; gold nanoparticle; c-Myc siRNA; anti-cancer therapy
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MDPI and ACS Style

Mao, W.; Lee, S.; Shin, J.U.; Yoo, H.S. Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy. Pharmaceutics 2020, 12, 261.

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