Self-Assembled Disulfide Bond Bearing Paclitaxel—Camptothecin Prodrug Nanoparticle for Lung Cancer Therapy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of PTX-S-S-CPT
2.3. Characterization of CPT-S-S-PTX
2.4. Assembly of Three-Dimensional Microfluidic Chip
2.5. Preparation of Nanoparticles
2.6. Characterization of the Nanoparticles
2.7. In Vitro Release of PTX and CPT from the Nanoparticles
2.8. Cell Assays
2.8.1. Cell Culture and Maintenance
2.8.2. Cytotoxicity Assay
2.8.3. Cellular Uptake Evaluation
3. Results
3.1. Characterization of CPT-S-S-PTX Prodrug
3.2. Characterization of Prodrug-Assembled Nanoparticles
3.3. In Vitro Release of CPT from the CPT-S-S-PTX Nanoparticles
3.4. In Vitro Cell Viability Assay
3.5. Cell Uptake
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gao, J.; Ma, X.; Zhang, L.; Yan, J.; Cui, H.; Zhang, Y.; Wang, D.; Zhang, H. Self-Assembled Disulfide Bond Bearing Paclitaxel—Camptothecin Prodrug Nanoparticle for Lung Cancer Therapy. Pharmaceutics 2020, 12, 1169. https://doi.org/10.3390/pharmaceutics12121169
Gao J, Ma X, Zhang L, Yan J, Cui H, Zhang Y, Wang D, Zhang H. Self-Assembled Disulfide Bond Bearing Paclitaxel—Camptothecin Prodrug Nanoparticle for Lung Cancer Therapy. Pharmaceutics. 2020; 12(12):1169. https://doi.org/10.3390/pharmaceutics12121169
Chicago/Turabian StyleGao, Jingyan, Xiaodong Ma, Lirong Zhang, Jiaqi Yan, Huaguang Cui, Yuezhou Zhang, Dongqing Wang, and Hongbo Zhang. 2020. "Self-Assembled Disulfide Bond Bearing Paclitaxel—Camptothecin Prodrug Nanoparticle for Lung Cancer Therapy" Pharmaceutics 12, no. 12: 1169. https://doi.org/10.3390/pharmaceutics12121169