Next Article in Journal
Systemic Design and Evaluation of Ticagrelor-Loaded Nanostructured Lipid Carriers for Enhancing Bioavailability and Antiplatelet Activity
Next Article in Special Issue
Use of nPSi-βCD Composite Microparticles for the Controlled Release of Caffeic Acid and Pinocembrin, Two Main Polyphenolic Compounds Found in a Chilean Propolis
Previous Article in Journal
Octreotide Conjugates for Tumor Targeting and Imaging
Previous Article in Special Issue
Modulation of the Release of a Non-Interacting Low Solubility Drug from Chitosan Pellets Using Different Pellet Size, Composition and Numerical Optimization
Open AccessArticle

Thermally-Responsive Loading and Release of Elastin-Like Polypeptides from Contact Lenses

1
Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90033, USA
2
Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
3
Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA
4
Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
*
Author to whom correspondence should be addressed.
Authors contributed equally to this research.
Pharmaceutics 2019, 11(5), 221; https://doi.org/10.3390/pharmaceutics11050221
Received: 25 March 2019 / Revised: 20 April 2019 / Accepted: 24 April 2019 / Published: 7 May 2019
(This article belongs to the Special Issue Functional Polymers for Controlled Drug Release)
Contact lenses are widely prescribed for vision correction, and as such they are an attractive platform for drug delivery to the anterior segment of the eye. This manuscript explores a novel strategy to drive the reversible adsorption of peptide-based therapeutics using commercially available contact lenses. To accomplish this, thermo-sensitive elastin-like polypeptides (ELPs) alone or tagged with a candidate ocular therapeutic were characterized. For the first time, this manuscript demonstrates that Proclear CompatiblesTM contact lenses are a suitable platform for ELP adsorption. Two rhodamine-labelled ELPs, V96 (thermo-sensitive) and S96 (thermo-insensitive), were employed to test temperature-dependent association to the contact lenses. During long-term release into solution, ELP coacervation significantly modulated the release profile whereby more than 80% of loaded V96 retained with a terminal half-life of ~4 months, which was only 1–4 days under solubilizing conditions. A selected ocular therapeutic candidate lacritin-V96 fusion (LV96), either free or lens-bound LV96, was successfully transferred to HCE-T cells. These data suggest that ELPs may be useful to control loading or release from certain formulations of contact lenses and present a potential for this platform to deliver a biologically active peptide to the ocular surface via contact lenses. View Full-Text
Keywords: elastin-like polypeptide (ELPs); contact lens; lacritin; protein therapeutics; drug delivery elastin-like polypeptide (ELPs); contact lens; lacritin; protein therapeutics; drug delivery
Show Figures

Graphical abstract

MDPI and ACS Style

Wang, W.; Lee, C.; Pastuszka, M.; Laurie, G.W.; MacKay, J.A. Thermally-Responsive Loading and Release of Elastin-Like Polypeptides from Contact Lenses. Pharmaceutics 2019, 11, 221.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop