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Pharmaceutics 2019, 11(2), 95; https://doi.org/10.3390/pharmaceutics11020095

In Vitro Methods to Study Colon Release: State of the Art and An Outlook on New Strategies for Better In-Vitro Biorelevant Release Media

1
Department of Food technology engineering and nutrition, Lund University, P.O. Box 124, 221 00 Lund, Sweden
2
Ferring International PharmaScience Center (IPC), Kay Fiskers Plads 11, 2300 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Received: 29 January 2019 / Revised: 18 February 2019 / Accepted: 21 February 2019 / Published: 22 February 2019
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Abstract

The primary focus of this review is a discussion regarding in vitro media for colon release, but we also give a brief overview of colon delivery and the colon microbiota as a baseline for this discussion. The large intestine is colonized by a vast number of bacteria, approximately 1012 per gram of intestinal content. The microbial community in the colon is complex and there is still much that is unknown about its composition and the activity of the microbiome. However, it is evident that this complex microbiota will affect the release from oral formulations targeting the colon. This includes the release of active drug substances, food supplements, and live microorganisms, such as probiotic bacteria and bacteria used for microbiota transplantations. Currently, there are no standardized colon release media, but researchers employ in vitro models representing the colon ranging from reasonable simple systems with adjusted pH with or without key enzymes to the use of fecal samples. In this review, we present the pros and cons for different existing in vitro models. Furthermore, we summarize the current knowledge of the colonic microbiota composition which is of importance to the fermentation capacity of carbohydrates and suggest a strategy to choose bacteria for a new more standardized in vitro dissolution medium for the colon. View Full-Text
Keywords: in vitro systems; colon delivery; colon microbiota in vitro systems; colon delivery; colon microbiota
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Wahlgren, M.; Axenstrand, M.; Håkansson, Å.; Marefati, A.; Lomstein Pedersen, B. In Vitro Methods to Study Colon Release: State of the Art and An Outlook on New Strategies for Better In-Vitro Biorelevant Release Media. Pharmaceutics 2019, 11, 95.

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