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Article

Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery

1
Department of Chemical Engineering, School of Engineering, Nazarbayev University, Nur-Sultan 010000, Kazakhstan
2
Department of Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan
3
School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UK
4
ANAMAD Ltd., Falmer, Brighton BN1 9SB, UK
5
Chuiko Institute of Surface Chemistry, Kyiv 01364, Ukraine
6
School of Medicine, Nazarbayev University, Nur-Sultan 010000, Kazakhstan
*
Author to whom correspondence should be addressed.
Pharmaceutics 2019, 11(12), 650; https://doi.org/10.3390/pharmaceutics11120650
Received: 10 October 2019 / Revised: 11 November 2019 / Accepted: 20 November 2019 / Published: 4 December 2019
Macroporous scaffolds composed of chitosan (CHI), hydroxyapatite (HA), heparin (Hep), and polyvinyl alcohol (PVA) were prepared with a glutaraldehyde (GA) cross-linker by cryogelation. Addition of PVA to the reaction mixture slowed down the formation of a polyelectrolyte complex (PEC) between CHI and Hep, which allowed more thorough mixing, and resulted in the development of the homogeneous matrix structure. Freezing of the CHI-HA-GA and PVA-Hep-GA mixture led to the formation of a non-stoichiometric PEC between oppositely charged groups of CHI and Hep, which caused further efficient immobilization of bone morphogenic protein 2 (BMP-2) possible due to electrostatic interactions. It was shown that the obtained cryogel matrix released BMP-2 and supported the differentiation of rat bone marrow mesenchymal stem cells (rat BMSCs) into the osteogenic lineage. Rat BMSCs attached to cryogel loaded with BMP-2 and expressed osteocalcin in vitro. Obtained composite cryogel with PEC may have high potential for bone regeneration and tissue engineering applications. View Full-Text
Keywords: biomaterials; cryogel; drug delivery; growth factors; tissue engineering; bone regeneration biomaterials; cryogel; drug delivery; growth factors; tissue engineering; bone regeneration
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MDPI and ACS Style

Sultankulov, B.; Berillo, D.; Kauanova, S.; Mikhalovsky, S.; Mikhalovska, L.; Saparov, A. Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery. Pharmaceutics 2019, 11, 650. https://doi.org/10.3390/pharmaceutics11120650

AMA Style

Sultankulov B, Berillo D, Kauanova S, Mikhalovsky S, Mikhalovska L, Saparov A. Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery. Pharmaceutics. 2019; 11(12):650. https://doi.org/10.3390/pharmaceutics11120650

Chicago/Turabian Style

Sultankulov, Bolat; Berillo, Dmitriy; Kauanova, Sholpan; Mikhalovsky, Sergey; Mikhalovska, Lyuba; Saparov, Arman. 2019. "Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery" Pharmaceutics 11, no. 12: 650. https://doi.org/10.3390/pharmaceutics11120650

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