Highly Water-Soluble Solid Dispersions of Honokiol: Preparation, Solubility, and Bioavailability Studies and Anti-Tumor Activity Evaluation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of the HK SD Formulation
2.3. Characterization of the HK SD Formulations
2.3.1. Scanning Electron Microscopy (SEM)
2.3.2. Fourier Transform Infrared Spectroscopy (FTIR)
2.3.3. X-Ray Diffractometry (XRD)
2.3.4. Differential Scanning Calorimetry (DSC).
2.3.5. Gas Chromatography (GC) Measurement
2.4. Solubility Tests of the HK SD Formulations
2.4.1. High Performance Liquid Chromatograph (HPLC) Method for HK
2.4.2. Solubility Tests
2.5. Dissolution Tests
2.6. In Vivo Pharmacokinetic Study
2.6.1. Animal Dosing
2.6.2. Determination of HK Content in Plasma Samples
2.7. MTT Assay
2.8. Statistical Analysis
3. Results and Discussion
3.1. Solubility Enhancement
3.1.1. Solubility of Different Solid Dispersions of HK
3.1.2. Solubility of PMs and SD of HK
3.2. Characterization of HK Formulations
3.2.1. SEM Results
3.2.2. Physicochemical Properties Characterization
3.2.3. Residual Solvent Determination
3.3. Dissolution Study
3.4. Pharmacokinetic Behavior of HK Formulations
3.5. MTT Study
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Pharmacokinetic Parameters | Free HK | Physical Mixture of HK and PLX | HK–PLX (1:4) SD |
---|---|---|---|
Cmax (ng/mL) | 159.02 ± 5.65 | 443.81 ± 9.14 * | 1109.87 ± 7.24 * |
Tmax (h) | 2 ± 0.15 | 0.75 ± 0.09 * | 0.5 ± 0.08 * |
t1/2 (h) | 0.74 ± 0.15 | 0.51 ± 0.12 | 0.37 ± 0.05 * |
MRT(0–t) (h) | 3.93 ± 1.25 | 6.94 ± 2.22 * | 5.70 ± 0.76 * |
MRT(0–∞) (h) | 5.11 ± 2.13 | 12.15 ± 3.15 * | 6.44 ± 1.15 |
AUC(0–t) (ng/mL·h) | 580.45 ± 11.15 | 848.34 ± 12.24 * | 2558.22 ± 8.15 * |
AUC(0–∞) (ng/mL·h) | 637.91 ± 13.42 | 1004.11 ± 15.38 * | 2588.70 ± 10.54 * |
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Wang, L.; Wu, W.; Wang, L.; Wang, L.; Zhao, X. Highly Water-Soluble Solid Dispersions of Honokiol: Preparation, Solubility, and Bioavailability Studies and Anti-Tumor Activity Evaluation. Pharmaceutics 2019, 11, 573. https://doi.org/10.3390/pharmaceutics11110573
Wang L, Wu W, Wang L, Wang L, Zhao X. Highly Water-Soluble Solid Dispersions of Honokiol: Preparation, Solubility, and Bioavailability Studies and Anti-Tumor Activity Evaluation. Pharmaceutics. 2019; 11(11):573. https://doi.org/10.3390/pharmaceutics11110573
Chicago/Turabian StyleWang, Li, Weiwei Wu, Lingling Wang, Lu Wang, and Xiuhua Zhao. 2019. "Highly Water-Soluble Solid Dispersions of Honokiol: Preparation, Solubility, and Bioavailability Studies and Anti-Tumor Activity Evaluation" Pharmaceutics 11, no. 11: 573. https://doi.org/10.3390/pharmaceutics11110573
APA StyleWang, L., Wu, W., Wang, L., Wang, L., & Zhao, X. (2019). Highly Water-Soluble Solid Dispersions of Honokiol: Preparation, Solubility, and Bioavailability Studies and Anti-Tumor Activity Evaluation. Pharmaceutics, 11(11), 573. https://doi.org/10.3390/pharmaceutics11110573