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Pharmaceutics 2018, 10(3), 94; https://doi.org/10.3390/pharmaceutics10030094

Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?

1
Institute of Pharma Technology, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132 Muttenz, Switzerland
2
LS Instruments, Passage du Cardinal 1, 1700 Fribourg, Switzerland
*
Author to whom correspondence should be addressed.
Received: 1 June 2018 / Revised: 10 July 2018 / Accepted: 13 July 2018 / Published: 17 July 2018
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Abstract

Background: Dynamic light scattering (DLS) is an important tool to characterize colloidal systems and adequate sizing is particularly critical in the field of protein formulations. Among the different factors that can influence the measurement result, the effect of laser power has so far not been studied thoroughly. Methods: The sensitivity of a DLS instrument was first considered on a theoretical level, followed by experiments using DLS instruments, equipped with two different lasers of (nominal) 45 mW, and 100 mW, respectively. This work analyzes dilute colloidal dispersions of lysozyme as model protein. Results: Theoretical findings agreed with experiments in that only enhanced laser power of 100 mW laser allowed measuring a 0.1 mg/mL protein dispersion in a reliable manner. Results confirmed the usefulness of the presented theoretical considerations in improving a general understanding of the limiting factors in DLS. Conclusions: Laser power is a critical aspect regarding adequate colloidal analysis by DLS. Practical guidance is provided to help scientists specifically with measuring dilute samples to choose both an optimal instrument configuration as well as a robust experimental procedure. View Full-Text
Keywords: protein formulation; protein aggregation; dynamic light scattering (DSL); laser power; lysozyme protein formulation; protein aggregation; dynamic light scattering (DSL); laser power; lysozyme
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Aleandri, S.; Vaccaro, A.; Armenta, R.; Völker, A.C.; Kuentz, M. Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power? Pharmaceutics 2018, 10, 94.

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