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Metagenomic Approaches to Assess Bacteriophages in Various Environmental Niches

School of Microbiology, University College Cork, Cork T12 YT20, Ireland
APC Microbiome Institute, University College Cork, Cork T12 YT20, Ireland
Friesland Campina, Amersfoort 3800 BN, The Netherlands
Author to whom correspondence should be addressed.
Academic Editors: Tessa E. F. Quax, Matthias G. Fischer and Laurent Debarbieux
Viruses 2017, 9(6), 127;
Received: 31 March 2017 / Revised: 18 May 2017 / Accepted: 19 May 2017 / Published: 24 May 2017
(This article belongs to the Special Issue Viruses of Microbes)
Bacteriophages are ubiquitous and numerous parasites of bacteria and play a critical evolutionary role in virtually every ecosystem, yet our understanding of the extent of the diversity and role of phages remains inadequate for many ecological niches, particularly in cases in which the host is unculturable. During the past 15 years, the emergence of the field of viral metagenomics has drastically enhanced our ability to analyse the so-called viral ‘dark matter’ of the biosphere. Here, we review the evolution of viral metagenomic methodologies, as well as providing an overview of some of the most significant applications and findings in this field of research. View Full-Text
Keywords: virome; phage; marine; microbiota virome; phage; marine; microbiota
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Hayes, S.; Mahony, J.; Nauta, A.; Van Sinderen, D. Metagenomic Approaches to Assess Bacteriophages in Various Environmental Niches. Viruses 2017, 9, 127.

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