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Open AccessArticle

MicroRNA and mRNA Dysregulation in Astrocytes Infected with Zika Virus

1
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada
2
Molecular Patho Biology, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
3
Viral Diseases Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
4
Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada; Winnipeg, MB R3E 3R2, Canada, [email protected]
5
Medical Microbiology, Public Health Ontario Laboratory, Toronto, ON M5G 1M1, Canada
6
Research Institute in Oncology and Hematology, Cancer Care Manitoba, Winnipeg, MB R3E 0V9, Canada
7
Viral Exanthemata and STD, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
8
Viral Zoonoses, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
9
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
10
Infectious Diseases Research Centre, Université Laval, Quebec, QC G1V 4G2, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2017, 9(10), 297; https://doi.org/10.3390/v9100297
Received: 11 September 2017 / Revised: 30 September 2017 / Accepted: 10 October 2017 / Published: 14 October 2017
(This article belongs to the Section Animal Viruses)
The Zika virus (ZIKV) epidemic is an ongoing public health concern. ZIKV is a flavivirus reported to be associated with microcephaly, and recent work in animal models demonstrates the ability of the virus to cross the placenta and affect fetal brain development. Recent findings suggest that the virus preferentially infects neural stem cells and thereby deregulates gene expression, cell cycle progression, and increases cell death. However, neuronal stem cells are not the only brain cells that are susceptible to ZIKV and infection of other brain cells may contribute to disease progression. Herein, we characterized ZIKV replication in astrocytes, and profiled temporal changes in host microRNAs (miRNAs) and transcriptomes during infection. We observed the deregulation of numerous processes known to be involved in flavivirus infection, including genes involved in the unfolded protein response pathway. Moreover, a number of miRNAs were upregulated, including miR-30e-3p, miR-30e-5p, and, miR-17-5p, which have been associated with other flavivirus infections. This study highlights potential miRNAs that may be of importance in ZIKV pathogenesis. View Full-Text
Keywords: Zika virus; microRNA; human astrocytes; host response; pathogenesis; flavivirus Zika virus; microRNA; human astrocytes; host response; pathogenesis; flavivirus
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Kozak, R.A.; Majer, A.; Biondi, M.J.; Medina, S.J.; Goneau, L.W.; Sajesh, B.V.; Slota, J.A.; Zubach, V.; Severini, A.; Safronetz, D.; Hiebert, S.L.; Beniac, D.R.; Booth, T.F.; Booth, S.A.; Kobinger, G.P. MicroRNA and mRNA Dysregulation in Astrocytes Infected with Zika Virus. Viruses 2017, 9, 297.

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