Next Article in Journal
Echovirus 6 Infects Human Exocrine and Endocrine Pancreatic Cells and Induces Pro-Inflammatory Innate Immune Response
Previous Article in Journal
Virus Escape and Manipulation of Cellular Nonsense-Mediated mRNA Decay
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 9
Issue 1
10.3390/v9010023
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Feline Panleucopenia Virus NS2 Suppresses the Host IFN-β Induction by Disrupting the Interaction between TBK1 and STING

by
Hongtao Kang
1,†,
Dafei Liu
1,†,
Jin Tian
1,
Xiaoliang Hu
1,
Xiaozhan Zhang
1,
Hang Yin
2,
Hongxia Wu
1,
Chunguo Liu
1,
Dongchun Guo
1,
Zhijie Li
1,
Qian Jiang
1,
Jiasen Liu
1 and
Liandong Qu
1,*
1
Division of Zoonosis of Natural Foci, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping road, Xiangfang District, Harbin 150000, China
2
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Viruses 2017, 9(1), 23; https://doi.org/10.3390/v9010023
Submission received: 23 November 2016 / Revised: 17 January 2017 / Accepted: 18 January 2017 / Published: 23 January 2017
(This article belongs to the Section Animal Viruses)
Browse Figures
Versions Notes

Abstract

Feline panleucopenia virus (FPV) is a highly infectious pathogen that causes severe diseases in pets, economically important animals and wildlife in China. Although FPV was identified several years ago, little is known about how it overcomes the host innate immunity. In the present study, we demonstrated that infection with the FPV strain Philips-Roxane failed to activate the interferon β (IFN-β) pathway but could antagonize the induction of IFN stimulated by Sendai virus (SeV) in F81 cells. Subsequently, by screening FPV nonstructural and structural proteins, we found that only nonstructural protein 2 (NS2) significantly suppressed IFN expression. We demonstrated that the inhibition of SeV-induced IFN-β production by FPV NS2 depended on the obstruction of the IFN regulatory factor 3 (IRF3) signaling pathway. Further, we verified that NS2 was able to target the serine/threonine-protein kinase TBK1 and prevent it from being recruited by stimulator of interferon genes (STING) protein, which disrupted the phosphorylation of the downstream protein IRF3. Finally, we identified that the C-terminus plus the coiled coil domain are the key domains of NS2 that are required for inhibiting the IFN pathway. Our study has yielded strong evidence for the FPV mechanisms that counteract the host innate immunity.
Keywords: Feline panleucopenia virus (FPV); Nonstructural protein 2 (NS2); interferon β (IFN-β) Feline panleucopenia virus (FPV); Nonstructural protein 2 (NS2); interferon β (IFN-β)

Share and Cite

MDPI and ACS Style

Kang, H.; Liu, D.; Tian, J.; Hu, X.; Zhang, X.; Yin, H.; Wu, H.; Liu, C.; Guo, D.; Li, Z.; et al. Feline Panleucopenia Virus NS2 Suppresses the Host IFN-β Induction by Disrupting the Interaction between TBK1 and STING. Viruses 2017, 9, 23. https://doi.org/10.3390/v9010023

AMA Style

Kang H, Liu D, Tian J, Hu X, Zhang X, Yin H, Wu H, Liu C, Guo D, Li Z, et al. Feline Panleucopenia Virus NS2 Suppresses the Host IFN-β Induction by Disrupting the Interaction between TBK1 and STING. Viruses. 2017; 9(1):23. https://doi.org/10.3390/v9010023

Chicago/Turabian Style

Kang, Hongtao, Dafei Liu, Jin Tian, Xiaoliang Hu, Xiaozhan Zhang, Hang Yin, Hongxia Wu, Chunguo Liu, Dongchun Guo, Zhijie Li, and et al. 2017. "Feline Panleucopenia Virus NS2 Suppresses the Host IFN-β Induction by Disrupting the Interaction between TBK1 and STING" Viruses 9, no. 1: 23. https://doi.org/10.3390/v9010023

APA Style

Kang, H., Liu, D., Tian, J., Hu, X., Zhang, X., Yin, H., Wu, H., Liu, C., Guo, D., Li, Z., Jiang, Q., Liu, J., & Qu, L. (2017). Feline Panleucopenia Virus NS2 Suppresses the Host IFN-β Induction by Disrupting the Interaction between TBK1 and STING. Viruses, 9(1), 23. https://doi.org/10.3390/v9010023

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop