Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells

Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low viral titers are obtained during the propagation of PEDV in vitro; this represents a challenge to molecular analyses of the virus and vaccine development. In this study, we successfully isolated a PEDV isolate (strain NJ) from clinical samples collected during a recent outbreak of diarrhea in piglets in China, using porcine intestinal epithelial cells (IEC). We found that the isolate was better adapted to growth in IECs than in Vero cells, and the titer of the IEC cultures was 10^4.5 TCID₅₀/0.1 mL at passage 45. Mutations in the S protein increased with the viral passage and the mutations tended towards attenuation. Viral challenge showed that the survival of IEC-adapted cultures was higher at the 45th passage than at the 5th passage. The use of IECs to isolate and propagate PEDV provides an effective approach for laboratory-based diagnosis of PEDV, as well as studies of the epidemiological characteristics and molecular biology of this virus. View Full-Text

Keywords: porcine epidemic diarrhea virus (PEDV) NJ strain; porcine intestinal epithelial cells; isolation and identification

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MDPI and ACS Style

Shi, W.; Jia, S.; Zhao, H.; Yin, J.; Wang, X.; Yu, M.; Ma, S.; Wu, Y.; Chen, Y.; Fan, W.; Xu, Y.; Li, Y. Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells. Viruses 2017, 9, 19. https://doi.org/10.3390/v9010019

AMA Style

Shi W, Jia S, Zhao H, Yin J, Wang X, Yu M, Ma S, Wu Y, Chen Y, Fan W, Xu Y, Li Y. Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells. Viruses. 2017; 9(1):19. https://doi.org/10.3390/v9010019

Chicago/Turabian Style

Shi, Wen, Shuo Jia, Haiyuan Zhao, Jiyuan Yin, Xiaona Wang, Meiling Yu, Sunting Ma, Yang Wu, Ying Chen, Wenlu Fan, Yigang Xu, and Yijing Li. 2017. "Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells" Viruses 9, no. 1: 19. https://doi.org/10.3390/v9010019

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Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells

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