The Effect of MicroRNA bantam on Baculovirus AcMNPV Infection in Vitro and in Vivo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells, Viruses, MicroRNA Mimic, Inhibitor and AntagomiR
2.2. Transfection and Infection
2.3. Real-Time PCR
2.4. Insect Experiments
3. Results
3.1. bantam Level after Infection
3.2. The Effect of bantam Mimic and Inhibitor on AcMNPV Replication in Sf9 Cells
3.3. The Effect of bantam antagomiR in AcMNPV Infection in Spodoptera larvae
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
MOI | multiplicity of infection |
AcMNPV | Autographa californica multicapsid nucleopolyhedrovirus |
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Target | Type | Sequence (5′–3′) |
---|---|---|
bantam | primer assay | CTTTCTGAGATCATTGTGAAAG |
miR-750 | primer assay | CAGTTGCCAGATCTATCTTTC |
U6 (B. mori) | primer assay | AGAGACGATTAGCATGGCCC |
ie-0 | upper primer | CATATTCGTGCGAGGCAACG |
lower primer | CGAGTTGACGCTTGCCAAAA | |
ie-1 | upper primer | TTTTAACGCGTCGTACACCA |
lower primer | GTTGACGCTTGCCAAAAAGT | |
ie2 | upper primer | AGCGAAGAAAACGTGCAGAT |
lower primer | CTCCGACGCAATGTTATCCT | |
lef-1 | upper primer | TTCCGCATATGCAAGATTCA |
lower primer | ACCACATCCACCAGTTCCAT | |
lef-3 | upper primer | AACCCCCATGAGCGACTATTTG |
lower primer | ACCGCTGTTGATTTTCTTGTA | |
lef-8 | upper primer | AATGGCTACTCTGTGGCGGTAA |
lower primer | TTACCGCCACAGAGTAGCCATT | |
egt | upper primer | ACCGTTTCCAGCGATCAACT |
lower primer | TAGAGGCGGAAACGTTGCTT | |
me53 | upper primer | CGCATCTCTCCGCCTAAACA |
lower primer | GCGGGCAAACCTTCAAACTT | |
p143 | upper primer | AAGTATTTGCCCGAGGAC |
lower primer | ATTTTGGCGATGTGATAAGT | |
p35 | upper primer | ACGACACGGGACTTTACGAG |
lower primer | GTTTTTCGACGCTTCGTTGT | |
vlf-1 | upper primer | CGTGCCAATTCCAACGGTTT |
lower primer | CAACTCAGCGTGGACGATCT | |
gp41 | upper primer | AGAGTTGGGACAGAGCAACG |
lower primer | GCGCCACCGTTGTAAAACTT | |
orf-1629 | upper primer | GTTAGGCACGGGAGAA |
lower primer | CGAAGCAGACGACCTT | |
p10 | upper primer | GACGCCGTTACGGAAACTAA |
lower primer | GTCTGGAAGATCCGGAACAA | |
egfp | upper primer | CGTAAACGGCCACAAGTTCAT |
lower primer | GGGTCAGCTTGCCGTAGGT | |
orf-75 | upper primer | GCTGGCAGTTGGTATGCTTC |
lower primer | ACTCAGTAGGCGACAGGTTG | |
β-tublin (B. mori) | upper primer | TTGCATTGGTACACTGGCGA |
lower primer | ACACCAGGTCGTTCATGTTGC |
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Shi, X.; Ran, Z.; Li, S.; Yin, J.; Zhong, J. The Effect of MicroRNA bantam on Baculovirus AcMNPV Infection in Vitro and in Vivo. Viruses 2016, 8, 136. https://doi.org/10.3390/v8050136
Shi X, Ran Z, Li S, Yin J, Zhong J. The Effect of MicroRNA bantam on Baculovirus AcMNPV Infection in Vitro and in Vivo. Viruses. 2016; 8(5):136. https://doi.org/10.3390/v8050136
Chicago/Turabian StyleShi, Xiaojie, Zihan Ran, Sisi Li, Juan Yin, and Jiang Zhong. 2016. "The Effect of MicroRNA bantam on Baculovirus AcMNPV Infection in Vitro and in Vivo" Viruses 8, no. 5: 136. https://doi.org/10.3390/v8050136