Optimization and Characterization of Candidate Strain for Coxsackievirus A16 Inactivated Vaccine
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Cells and CA16 Viruses
2.3. Determination of Viral Titer
2.4. Characterization of CA16 Viral Particles by Electron Microscopy
2.5. Neutralization Assay
2.6. Viral Genome Sequencing
2.7. Viral Growth Assay
2.8. Pilot Production of CA16 Vaccine
2.9. Mouse Immunization
2.10. Western Blot
2.11. Statistical Analysis
3. Results
3.1. Isolation and Characterization of CA16CC024 Vaccine Strain
3.2. Adaptation of CA16 Strains to Vero Cells
3.3. Biological Characterization of CA16CC024 Vaccine Strain
3.4. Cloning and Identification of CA16CC024 in Vero Cells
Characteristic | CC024 | CC-16 Passage | |||
---|---|---|---|---|---|
11th | 13th | 15th | |||
Genetic Homology | VP1 | - | - | - | - |
VP2 | - | - | - | - | |
VP3 | - | - | - | - | |
VP4 | Arg(51) | Lys | Lys | Lys |
3.5. Preliminary Upstream Process Development
3.6. Preliminary Downstream Process and Identification
3.7. Inactivated CA16CC-16 Vaccine Elicits Humoral Immunity in Mice
4. Discussion
5. Conclusion
Acknowledgments
Author Contributions
Conflicts of Interest
Appendix
References
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Li, J.; Liu, G.; Liu, X.; Yang, J.; Chang, J.; Zhang, W.; Yu, X.-F. Optimization and Characterization of Candidate Strain for Coxsackievirus A16 Inactivated Vaccine. Viruses 2015, 7, 3891-3909. https://doi.org/10.3390/v7072803
Li J, Liu G, Liu X, Yang J, Chang J, Zhang W, Yu X-F. Optimization and Characterization of Candidate Strain for Coxsackievirus A16 Inactivated Vaccine. Viruses. 2015; 7(7):3891-3909. https://doi.org/10.3390/v7072803
Chicago/Turabian StyleLi, Jingliang, Guanchen Liu, Xin Liu, Jiaxin Yang, Junliang Chang, Wenyan Zhang, and Xiao-Fang Yu. 2015. "Optimization and Characterization of Candidate Strain for Coxsackievirus A16 Inactivated Vaccine" Viruses 7, no. 7: 3891-3909. https://doi.org/10.3390/v7072803