Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China
1
Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China
2
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271018, China
3
Shandong Jianmu Biological Pharmaceutical Co., Ltd., Jinan, Shandong 250100, China
4
Animal Diseases Diagnostic Laboratory, Ohio Department of Agriculture, Reynoldsburg, OH 43068, USA
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Eric O. Freed
Viruses 2015, 7(3), 887-898; https://doi.org/10.3390/v7030887
Received: 10 January 2015 / Revised: 12 February 2015 / Accepted: 13 February 2015 / Published: 25 February 2015
(This article belongs to the Section Animal Viruses)
Abstract
Infection of poultry with diverse lineages of H5N2 avian influenza viruses has been documented for over three decades in different parts of the world, with limited outbreaks caused by this highly pathogenic avian influenza virus. In the present study, three avian H5N2 influenza viruses, A/chicken/Shijiazhuang/1209/2013, A/chicken/Chiping/0321/2014, and A/chicken/Laiwu/0313/2014, were isolated from chickens with clinical symptoms of avian influenza. Complete genomic and phylogenetic analyses demonstrated that all three isolates are novel recombinant viruses with hemagglutinin (HA) and matrix (M) genes derived from H5N1, and remaining genes derived from H9N2-like viruses. The HA cleavage motif in all three strains (PQIEGRRRKR/GL) is characteristic of a highly pathogenic avian influenza virus strain. These results indicate the occurrence of H5N2 recombination and highlight the importance of continued surveillance of the H5N2 subtype virus and reformulation of vaccine strains. View Full-TextKeywords:
avian influenza virus; H5N2; recombination; genomic characterization
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MDPI and ACS Style
Xu, H.; Meng, F.; Huang, D.; Sheng, X.; Wang, Y.; Zhang, W.; Chang, W.; Wang, L.; Qin, Z. Genomic and Phylogenetic Characterization of Novel, Recombinant H5N2 Avian Influenza Virus Strains Isolated from Vaccinated Chickens with Clinical Symptoms in China. Viruses 2015, 7, 887-898.