Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant
Abstract
:1. Introduction
2. Materials and Methods
2.1. Rotavirus Strains
2.2. RNA Extraction
2.3. RT-PCR
2.4. Nucleotide Sequencing
2.5. Determination of the 5’ and 3’ Ends
2.6. Nucleotide Sequence Analysis
2.7. Phylogenetic Analysis
3. Results
3.1. Complete Genome Sequencing of BE5028
Strain Name | VP7 | VP4 | VP6 | VP1 | VP2 | VP3 | NSP1 | NSP2 | NSP3 | NSP4 | NSP5 |
---|---|---|---|---|---|---|---|---|---|---|---|
RVA/Human-tc/USA/Wa/1974/G1P[8] | G1 | P[8] | I1 | R1 | C1 | M1 | A1 | N1 | T1 | E1 | H1 |
RVA/Human-tc/USA/DS-1/1976/G2P[4] | G2 | P[4] | I2 | R2 | C2 | M2 | A2 | N2 | T2 | E2 | H2 |
RVA/Human-tc/JPN/AU-1/1982/G3P[9] | G3 | P[9] | I3 | R3 | C3 | M3 | A3 | N3 | T3 | E3 | H3 |
RVA/Human-wt/BEL/BE5028/2012/G3P[14] | G3 | P[14] | I2 | R2 | C2 | M3 | A9 | N2 | T6 | E5 | H3 |
RVA/Human-wt/BEL/B4106/2000/G3P[14] | G3 | P[14] | I2 | R2 | C2 | M3 | A9 | N2 | T6 | E5 | H3 |
RVA/Rabbit-tc/ITA/30-96/1996/G3P[14] | G3 | P[14] | I2 | R2 | C2 | M3 | A9 | N2 | T6 | E5 | H3 |
RVA/Rabbit-tc/CHN/N5/1992/G3P[14] | G3 | P[14] | I17 | R3 | C3 | M3 | A9 | N1 | T1 | E3 | H2 |
RVA/Human-wt/AUS/RCH272/2012/G3P[14] | G3 | P[14] | I2 | R3 | C3 | M3 | A9 | N2 | T6 | E2 | H3 |
RVA/Rabbit-tc/NLD/K1130027/2011/G6P[11] | G6 | P[11] | I2 | R2 | C2 | M2 | A13 | N2 | T6 | E2 | H3 |
3.2. Phylogenetic Analysis
3.3. Duplication in NSP5 Segment
4. Discussion
Supplementary Files
Supplementary File 1Supplementary File 2Acknowledgments
Author Contributions
Conflicts of Interest
References
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Bonica, M.B.; Zeller, M.; Van Ranst, M.; Matthijnssens, J.; Heylen, E. Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant. Viruses 2015, 7, 844-856. https://doi.org/10.3390/v7020844
Bonica MB, Zeller M, Van Ranst M, Matthijnssens J, Heylen E. Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant. Viruses. 2015; 7(2):844-856. https://doi.org/10.3390/v7020844
Chicago/Turabian StyleBonica, Melisa Berenice, Mark Zeller, Marc Van Ranst, Jelle Matthijnssens, and Elisabeth Heylen. 2015. "Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant" Viruses 7, no. 2: 844-856. https://doi.org/10.3390/v7020844
APA StyleBonica, M. B., Zeller, M., Van Ranst, M., Matthijnssens, J., & Heylen, E. (2015). Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant. Viruses, 7(2), 844-856. https://doi.org/10.3390/v7020844