Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant
AbstractGroup A rotaviruses (RVA) are responsible for causing infantile diarrhea both in humans and animals. The molecular characteristics of lapine RVA strains are only studied to a limited extent and so far G3P and G3P were found to be the most common G/P-genotypes. During the 2012-2013 rotavirus season in Belgium, a G3P RVA strain was isolated from stool collected from a two-year-old boy. We investigated whether RVA/Human-wt/BEL/BE5028/2012/G3P is completely of lapine origin or the result of reassortment event(s). Phylogenetic analyses of all gene segments revealed the following genotype constellation: G3-P-I2-R2-C2-M3-A9-N2-T6-E5-H3 and indicated that BE5028 probably represents a rabbit to human interspecies transmission able to cause disease in a human child. Interestingly, BE5028 showed a close evolutionary relationship to RVA/Human-wt/BEL/B4106/2000/G3P, another lapine-like strain isolated in a Belgian child in 2000. The phylogenetic analysis of the NSP3 segment suggests the introduction of a bovine(-like) NSP3 into the lapine RVA population in the past 12 years. Sequence analysis of NSP5 revealed a head-to-tail partial duplication, combined with two short insertions and a deletion, indicative of the continuous circulation of this RVA lineage within the rabbit population. View Full-Text
Share & Cite This Article
Bonica, M.B.; Zeller, M.; Van Ranst, M.; Matthijnssens, J.; Heylen, E. Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant. Viruses 2015, 7, 844-856.
Bonica MB, Zeller M, Van Ranst M, Matthijnssens J, Heylen E. Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant. Viruses. 2015; 7(2):844-856.Chicago/Turabian Style
Bonica, Melisa B.; Zeller, Mark; Van Ranst, Marc; Matthijnssens, Jelle; Heylen, Elisabeth. 2015. "Complete Genome Analysis of a Rabbit Rotavirus Causing Gastroenteritis in a Human Infant." Viruses 7, no. 2: 844-856.