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Open AccessCommunication

Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses

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Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
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Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA
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Carbone Cancer Center, University of Wisconsin Madison, Madison, WI 53706, USA
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GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA
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PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Viruses 2015, 7(10), 5319-5327; https://doi.org/10.3390/v7102873
Received: 4 September 2015 / Revised: 23 September 2015 / Accepted: 24 September 2015 / Published: 13 October 2015
(This article belongs to the Section Animal Viruses)
Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies. View Full-Text
Keywords: influenza virus; reporter virus; multi-modal imaging; multiplicity reactivation influenza virus; reporter virus; multi-modal imaging; multiplicity reactivation
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MDPI and ACS Style

Tran, V.; Poole, D.S.; Jeffery, J.J.; Sheahan, T.P.; Creech, D.; Yevtodiyenko, A.; Peat, A.J.; Francis, K.P.; You, S.; Mehle, A. Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses. Viruses 2015, 7, 5319-5327.

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