Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecular Assembly of (DAF)2-FcAlexa488 on Beads: Equilibrium and Kinetic Parameters
2.2. Binding between DAF and SNV on Beads and Tanoue B Cells Is Governed by Comparable Equilibrium Dissociation Constants
Cell | DAF a | Total αvβ3 b |
---|---|---|
Vero E6 | 5.85 ± 1.37 × 103 i | 1.61 ± 0.38 × 106 |
2.54 ± 0.84 × 105 ii | ||
2.26 ± 0.28 × 105 iii | ||
Tanoue B | 9.29 ± 2.18 × 104 i | None detected |
Kinetic Binding Analysis
2.3. Binding Parameters Derived from Bead Platform Provide a Reasonable Model for Infection Inhibition
3. Experimental Section
3.1. Materials
3.2. Production of Sin Nombre Virus
3.3. Fluorescent Labeling of SNV
3.4. Cloning, Expression and Purification of (DAF)2-Fc (v2) from Nicotiana benthamiana.
3.5. Fluorescent Labeling of (DAF)2-Fc
3.6. Cell Culture
3.7. Confocal Microscopy Imaging
3.8. Cytometry Experiments
3.9. Supported Bilayer Membranes on Glass Beads
3.10. Equilibrium Binding of Molecular Assembly Components (DAF)2-Fc, and SNVR18 on Protein G Beads
3.11. Cell Surface Distribution of DAF and αvβ3 Integrins
3.12. Virus Binding to DAF Expressed on Tanoue B Cells
3.13. Infectivity Assays
3.14. Ligand Binding Kinetic Measurements
3.15. Kinetic Modeling of Virus Binding
4. Conclusions
Acknowledgements
Author Contributions
Conflicts of Interest
References and Notes
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Appendix A
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Buranda, T.; Swanson, S.; Bondu, V.; Schaefer, L.; Maclean, J.; Mo, Z.; Wycoff, K.; Belle, A.; Hjelle, B. Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces. Viruses 2014, 6, 1091-1111. https://doi.org/10.3390/v6031091
Buranda T, Swanson S, Bondu V, Schaefer L, Maclean J, Mo Z, Wycoff K, Belle A, Hjelle B. Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces. Viruses. 2014; 6(3):1091-1111. https://doi.org/10.3390/v6031091
Chicago/Turabian StyleBuranda, Tione, Scarlett Swanson, Virginie Bondu, Leah Schaefer, James Maclean, Zhenzhen Mo, Keith Wycoff, Archana Belle, and Brian Hjelle. 2014. "Equilibrium and Kinetics of Sin Nombre Hantavirus Binding at DAF/CD55 Functionalized Bead Surfaces" Viruses 6, no. 3: 1091-1111. https://doi.org/10.3390/v6031091