Identification of a Conserved Linear Epitope on the p54 Protein of African Swine Fever Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Expression of the p54 Protein
2.2. Animal Immunization of Strategy
2.3. Preparation of mAbs
2.4. Western Blotting Analysis
2.5. Indirect Immunofluorescence Assay (IFA)
2.6. Identification of Linear B-Cell Epitopes
2.7. Blocking ELISA Analysis of ASFV-Positive Sera
2.8. Analysis of the Spatial Structure of Epitopes
3. Results
3.1. The Generation of the Recombinant p54 Protein
3.2. Generation and Characterization of Anti-p54 mAbs
3.3. Identifying the Epitope Recognized by the p54 mAbs
3.4. Key Residues of Epitope for Recognition by p54 mAb
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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He, K.; Wu, Y.; Su, Z.; Zeng, Y.; Ye, G.; Wu, Q.; Li, L.; Zhang, A. Identification of a Conserved Linear Epitope on the p54 Protein of African Swine Fever Virus. Viruses 2025, 17, 823. https://doi.org/10.3390/v17060823
He K, Wu Y, Su Z, Zeng Y, Ye G, Wu Q, Li L, Zhang A. Identification of a Conserved Linear Epitope on the p54 Protein of African Swine Fever Virus. Viruses. 2025; 17(6):823. https://doi.org/10.3390/v17060823
Chicago/Turabian StyleHe, Kuijing, Yue Wu, Zhipeng Su, Yue Zeng, Guishan Ye, Qi Wu, Long Li, and Anding Zhang. 2025. "Identification of a Conserved Linear Epitope on the p54 Protein of African Swine Fever Virus" Viruses 17, no. 6: 823. https://doi.org/10.3390/v17060823
APA StyleHe, K., Wu, Y., Su, Z., Zeng, Y., Ye, G., Wu, Q., Li, L., & Zhang, A. (2025). Identification of a Conserved Linear Epitope on the p54 Protein of African Swine Fever Virus. Viruses, 17(6), 823. https://doi.org/10.3390/v17060823