The Prevalence, Seroprevalence, and Risk Factors of Tick-Borne Encephalitis Virus in Dogs in Lithuania, a Highly Endemic State
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Data of Anamnesis
2.2. Sample Analysis with ELISA, VNT, and RT-nPCR
2.3. Virus Isolation
2.4. Statistical Analysis
3. Results
3.1. Prevalence of TBEV and TBEV-Specific Antibodies in Dogs
3.2. Risk Factors for Clinical TBE Infection in Dogs and Lethal Outcomes
3.3. TBEV Seroprevalence and Associations with Clinical Symptoms and the Presence of other Tick-Borne Diseases
3.4. TBEV RNA Prevalence in Ticks, Collected from Dogs
3.5. Virus Isolation from PCR-Positive Dogs’ Serum and CSF Samples
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Simkute, E.; Pautienius, A.; Grigas, J.; Urbute, P.; Stankevicius, A. The Prevalence, Seroprevalence, and Risk Factors of Tick-Borne Encephalitis Virus in Dogs in Lithuania, a Highly Endemic State. Viruses 2023, 15, 2265. https://doi.org/10.3390/v15112265
Simkute E, Pautienius A, Grigas J, Urbute P, Stankevicius A. The Prevalence, Seroprevalence, and Risk Factors of Tick-Borne Encephalitis Virus in Dogs in Lithuania, a Highly Endemic State. Viruses. 2023; 15(11):2265. https://doi.org/10.3390/v15112265
Chicago/Turabian StyleSimkute, Evelina, Arnoldas Pautienius, Juozas Grigas, Paulina Urbute, and Arunas Stankevicius. 2023. "The Prevalence, Seroprevalence, and Risk Factors of Tick-Borne Encephalitis Virus in Dogs in Lithuania, a Highly Endemic State" Viruses 15, no. 11: 2265. https://doi.org/10.3390/v15112265
APA StyleSimkute, E., Pautienius, A., Grigas, J., Urbute, P., & Stankevicius, A. (2023). The Prevalence, Seroprevalence, and Risk Factors of Tick-Borne Encephalitis Virus in Dogs in Lithuania, a Highly Endemic State. Viruses, 15(11), 2265. https://doi.org/10.3390/v15112265