Viral Metagenomics Reveals Diverse Viruses in Tissue Samples of Diseased Pigs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Preparation
2.2. Viral Nucleic Acid Extraction
2.3. Library Construction and Bioinformatics Analysis
2.4. Phylogenetic Analysis
2.5. Nucleotide Sequence Accession Number
3. Results
3.1. Viral Metagenomic Overview
3.2. Classical Swine Fever Virus
3.3. Porcine Reproductive and Respiratory Syndrome Virus
3.4. Porcine Circovirus Type 2
3.5. Porcine Parvovirus
3.6. Other Viruses That Only Obtained Short Fragments
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, S.; Zhang, D.; Ji, Z.; Zhang, Y.; Wang, Y.; Chen, X.; He, Y.; Lu, X.; Li, R.; Guo, Y.; et al. Viral Metagenomics Reveals Diverse Viruses in Tissue Samples of Diseased Pigs. Viruses 2022, 14, 2048. https://doi.org/10.3390/v14092048
Yang S, Zhang D, Ji Z, Zhang Y, Wang Y, Chen X, He Y, Lu X, Li R, Guo Y, et al. Viral Metagenomics Reveals Diverse Viruses in Tissue Samples of Diseased Pigs. Viruses. 2022; 14(9):2048. https://doi.org/10.3390/v14092048
Chicago/Turabian StyleYang, Shixing, Dianqi Zhang, Zexuan Ji, Yuyang Zhang, Yan Wang, Xu Chen, Yumin He, Xiang Lu, Rong Li, Yufei Guo, and et al. 2022. "Viral Metagenomics Reveals Diverse Viruses in Tissue Samples of Diseased Pigs" Viruses 14, no. 9: 2048. https://doi.org/10.3390/v14092048