Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Ethic Approval
2.2. Cell Lines
2.3. Virus Production, Titration and Infection
2.4. sEV Preparation
2.5. Nanoparticle Tracking Analysis (NTA)
2.6. Transmission Electron Microscopy and Immunolabeling Electron Microscopy
2.7. Western Blot
2.8. Quantitative Proteomic Analysis
2.8.1. Sample Preparation
2.8.2. NanoLC-MS/MS Analysis
2.8.3. Bioinformatics Data Analysis of Mass Spectrometry Raw Files
2.9. Functional Proteomic Data Analysis
2.10. Flow Cytometry Analysis
2.11. Immunofluorescence
2.12. Placental Histoculture
3. Results
3.1. HIPEC Infection by hCMV Leads to an Increase of sEV Secretion without Modifying Their General Features
3.2. sEVs Secreted by Infected HIPECs Harbor a Potential Proviral Protein Cargo
3.3. sEVs Are Efficiently Uptaken by Recipient MCR5 Cells
3.4. sEVs from hCMV-Infected HIPECs Potentialize Further Infection of Recipient MRC5 Cells
3.5. sEVs from hCMV-Infected HIPECs and Placental Tissues Enhance Infection of Human Neural Stem Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bergamelli, M.; Martin, H.; Aubert, Y.; Mansuy, J.-M.; Marcellin, M.; Burlet-Schiltz, O.; Hurbain, I.; Raposo, G.; Izopet, J.; Fournier, T.; et al. Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. Viruses 2022, 14, 2030. https://doi.org/10.3390/v14092030
Bergamelli M, Martin H, Aubert Y, Mansuy J-M, Marcellin M, Burlet-Schiltz O, Hurbain I, Raposo G, Izopet J, Fournier T, et al. Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. Viruses. 2022; 14(9):2030. https://doi.org/10.3390/v14092030
Chicago/Turabian StyleBergamelli, Mathilde, Hélène Martin, Yann Aubert, Jean-Michel Mansuy, Marlène Marcellin, Odile Burlet-Schiltz, Ilse Hurbain, Graça Raposo, Jacques Izopet, Thierry Fournier, and et al. 2022. "Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro" Viruses 14, no. 9: 2030. https://doi.org/10.3390/v14092030